3-一氯丙烷-1,2-二醇(3-MCPD),一种食源性污染物,被广泛认为是男性不育的主要原因。目前,确定改善/减少3-MCPD引起的男性生殖毒性的方法很重要。在我们的研究中,我们探讨了白藜芦醇(RSV)在减轻3-MCPD不良反应方面的潜在应用.以7周龄SD大鼠为动物模型,我们研究了3-MCPD和RSV对生殖功能的影响和潜在机制。3-MCPD的给药导致睾丸和附睾重量的显著减少,以及精子发生和组织学异常的中断。然而,与RSV和3-MCPD共同治疗减轻了这些不良反应。体外研究,RSV表现出逆转3-MCPD处理造成的Leydig和Sertoli细胞群下降的能力。机械上,RSV降低内质网应激(PARP),炎症小体激活(NLRP3),3-MCPD诱导的自噬介导的溶酶体功能障碍(p62和LC3BII)。此外,3-MCPD治疗增加了类固醇生成相关蛋白的表达水平,类固醇急性调节(StAR)和CYP11A1,但RSV标准化StAR表达。此外,3-MCPD诱导的促炎反应被RSV治疗抵消,随着细胞因子的减少和CD206表达的调节,巨噬细胞活化的标志.这些发现表明RSV减弱3-MCPD诱导的生殖毒性,强调其作为男性生殖健康佐剂的应用潜力。
3-Monochloropropane-1, 2-diol (3-MCPD), a food-borne contaminant, is widely regarded as the primary cause of male infertility. At present, identifying a method to improve/reduce the male reproductive toxicity caused by 3-MCPD is important. In our study, we explored the potential application of resveratrol (RSV) in mitigating the adverse effects of 3-MCPD. Using 7-week-old Sprague-Dawley (SD) rats as animal models, we investigated the impacts and underlying mechanisms of 3-MCPD and RSV on reproductive function. The administration of 3-MCPD led to significant reductions in testicular and epididymal weights, as well as disruptions in spermatogenesis and histological abnormalities. However, co-treatment with RSV and 3-MCPD mitigated these adverse effects. In vitro study, RSV exhibited the ability to reverse the decline in Leydig and Sertoli cell populations inflicted by 3-MCPD treatment. Mechanistically, RSV reduced endoplasmic reticulum stress (PARP), inflammasome activation (NLRP3), and autophagy-mediated lysosome dysfunction (p62 and LC3BII) induced by 3-MCPD. In addition, 3-MCPD treatment increased the expression level of steroidogenesis-related proteins, steroidogenic acute regulatory (StAR) and CYP11A1, but RSV normalized StAR expression. Moreover, 3-MCPD-induced pro-inflammatory responses were counteracted by RSV treatment, with the cytokine reduction and modulation of CD206 expression, a marker of macrophage activation. These findings indicate that RSV attenuates 3-MCPD-induced reproductive toxicity, highlighting its application potential as an adjuvant agent for male reproductive health.