%0 Journal Article
%T Determination of chloropropanol esters and glycidyl esters in nutritional foods by gas chromatography-tandem mass spectrometry based on acid hydrolysis and solid-phase extraction.
%A Li Y
%A Chen D
%A Fu W
%A Yang Y
%A Chen H
%A Ni L
%A Chen H
%A Jiang D
%A Zhang S
%A Gui R
%J Food Addit Contam Part A Chem Anal Control Expo Risk Assess
%V 41
%N 7
%D 2024 Jul 28
%M 38805241
%F 3.549
%R 10.1080/19440049.2024.2353796
%X This study presents a method based on acid transesterification and the purification by solid-phase extraction (SPE) coupled with gas chromatography-tandem mass spectrometry for quantifying 3- and 2-monochloropropanediol esters (3-MCPDE, 2-MCPDE) and glycidyl esters (GE) in nutritional foods. The fat was extracted by liquid-liquid extraction with petroleum ether and diethyl ether after the sample was hydrolysed with ammonia. Then the extract was purified by a SPE cartridge filled with the aminopropyl sorbents. It was demonstrated that the optimal elution volume for 3-MCPDE, 2-MCPDE and GE greatly depended on the sample matrix and varied from 6 to 12 mL for four different kinds of food matrices. All three analytes in the sample solution could be fully collected in the first 10-12 mL of eluate. By this way, monoacylglycerols commonly present in the samples were fully removed. Therefore, the overestimation of GE quantification was effectively eliminated. The modified analytical procedure was fully validated in a single laboratory and has been recommended as a Chinese Food Safety National Standard. In addition, two derivatisation agents, heptafluorobutyrylimidazole and phenylboronic acid, were proved to be equivalent in method accuracy and precision for the quantification of three analytes.