1,6-二磷酸果糖醛缩酶(FBA)基因家族存在于高等植物中,该家族的基因在植物生长发育中起着重要作用,以及对非生物胁迫的反应。然而,缺乏关于FBA基因家族及其在黄瓜中功能的系统报道。在这项研究中,我们鉴定了5个黄瓜FBA基因,命名为CsFBA1-5,随机分布在染色体上。涉及这些黄瓜FBA的系统发育分析,8种拟南芥FBA蛋白和8种番茄FBA蛋白,进行以评估它们的同源性。CsFBA分为两个分支。我们还分析了物理化学性质,主题组成,和黄瓜FBAs的基因结构。该分析强调了物理化学性质的差异,并揭示了CsFBA家族中高度保守的结构域。此外,为了进一步探索CsFBA家族的进化关系,我们构建了拟南芥和番茄的比较同势图,显示出很高的同源性,但在黄瓜基因组中只有一个片段重复事件。表达谱表明CsFBA基因家族对各种非生物胁迫有反应,包括低温,热,和盐。一起来看,本研究结果为了解黄瓜FBA基因在植物生长发育过程中的进化和功能特性研究提供了理论基础。
The fructose-1,6-bisphosphate aldolase (FBA) gene family exists in higher plants, with the genes of this family playing significant roles in plant growth and development, as well as response to abiotic stresses. However, systematic reports on the FBA gene family and its functions in cucumber are lacking. In this study, we identified five cucumber FBA genes, named CsFBA1-5, that are distributed randomly across chromosomes. Phylogenetic analyses involving these cucumber FBAs, alongside eight Arabidopsis FBA proteins and eight tomato FBA proteins, were conducted to assess their homology. The CsFBAs were grouped into two clades. We also analyzed the physicochemical properties, motif composition, and gene structure of the cucumber FBAs. This analysis highlighted differences in the physicochemical properties and revealed highly conserved domains within the CsFBA family. Additionally, to explore the evolutionary relationships of the CsFBA family further, we constructed comparative syntenic maps with Arabidopsis and tomato, which showed high homology but only one segmental duplication event within the cucumber genome. Expression profiles indicated that the CsFBA gene family is responsive to various abiotic stresses, including low temperature, heat, and salt. Taken together, the results of this study provide a theoretical foundation for understanding the evolution of and future research into the functional characterization of cucumber FBA genes during plant growth and development.