PDF(Pubmed)
Abstract：
Schima superba, commonly known as the Chinese guger tree, is highly adaptable and tolerant of poor soil conditions. It is one of the primary species forming the evergreen broad-leaved forests in southern China. Dirigent proteins (DIRs) play crucial roles in the synthesis of plant lignin and lignans, secondary metabolism, and response to adversity stress. However, research on the DIR gene family in S. superba is currently limited. This study identified 24 SsDIR genes, categorizing them into three subfamilies. These genes are unevenly distributed across 13 chromosomes, with 83% being intronless. Collinearity analysis indicated that tandem duplication played a more significant role in the expansion of the gene family compared to segmental duplication. Additionally, we analyzed the expression patterns of SsDIRs in different tissues of S. superba. The SsDIR genes exhibited distinct expression patterns across various tissues, with most being specifically expressed in the roots. Further screening identified SsDIR genes that may regulate drought stress, with many showing differential expression under drought stress conditions. In the promoter regions of SsDIRs, various cis-regulatory elements involved in developmental regulation, hormone response, and stress response were identified, which may be closely related to their diverse regulatory functions. This study will contribute to the further functional identification of SsDIR genes, providing insights into the biosynthetic pathways of lignin and lignans and the mechanisms of plant stress resistance.
摘要：
Schimasuperba,俗称中国古尔树，对恶劣的土壤条件具有很强的适应性和容忍度。它是中国南方常绿阔叶林的主要树种之一。分化蛋白(DIRs)在植物木质素和木脂素的合成中起着至关重要的作用,次生代谢,以及对逆境压力的反应。然而,目前，对S.superba中DIR基因家族的研究有限。这项研究确定了24个SsDIR基因，将它们分为三个亚科。这些基因在13条染色体上分布不均,83%是无内含子的。共线性分析表明，与分段复制相比，串联复制在基因家族的扩展中起着更重要的作用。此外,我们分析了SsDIR在S.superba不同组织中的表达模式。SsDIR基因在各种组织中表现出不同的表达模式，大多数在根部特别表达。进一步筛选确定了可能调节干旱胁迫的SsDIR基因，许多在干旱胁迫条件下表现出差异表达。在SsDIR的启动子区域,参与发育调节的各种顺式调节元件，激素反应，并确定了应激反应，这可能与其不同的监管功能密切相关。本研究将有助于SsDIR基因的进一步功能鉴定,提供对木质素和木脂素的生物合成途径以及植物抗逆机制的见解。
