PDF(Pubmed)
Abstract：
Alfin-like (AL) is a small plant-specific gene family characterized by a PHD-finger-like structural domain at the C-terminus and a DUF3594 structural domain at the N-terminus, and these genes play prominent roles in plant development and abiotic stress response. In this study, we conducted genome-wide identification and analyzed the AL protein family in Gossypium hirsutum cv. NDM8 to assess their response to various abiotic stresses for the first time. A total of 26 AL genes were identified in NDM8 and classified into four groups based on a phylogenetic tree. Moreover, cis-acting element analysis revealed that multiple phytohormone response and abiotic stress response elements were highly prevalent in AL gene promoters. Further, we discovered that the GhAL19 gene could negatively regulate drought and salt stresses via physiological and biochemical changes, gene expression, and the VIGS assay. The study found there was a significant increase in POD and SOD activity, as well as a significant change in MDA in VIGS-NaCl and VIGS-PEG plants. Transcriptome analysis demonstrated that the expression levels of the ABA biosynthesis gene (GhNCED1), signaling genes (GhABI1, GhABI2, and GhABI5), responsive genes (GhCOR47, GhRD22, and GhERFs), and the stress-related marker gene GhLEA14 were regulated in VIGS lines under drought and NaCl treatment. In summary, GhAL19 as an AL TF may negatively regulate tolerance to drought and salt by regulating the antioxidant capacity and ABA-mediated pathway.
摘要：
Alfin-like（AL）是一个小的植物特异性基因家族，其特征是在C末端具有PHD-finger-like结构域，在N末端具有DUF3594结构域，这些基因在植物发育和非生物胁迫反应中起着重要作用。在这项研究中,我们进行了全基因组鉴定，并分析了陆地棉cv中的AL蛋白家族。NDM8首次评估了它们对各种非生物胁迫的反应。在NDM8中鉴定出总共26个AL基因，并根据系统发育树分为四组。此外，顺式作用元件分析表明，多种植物激素反应和非生物应激反应元件在AL基因启动子中非常普遍。Further,我们发现GhAL19基因可以通过生理生化变化负调节干旱和盐胁迫,基因表达，和VIGS检测。研究发现POD和SOD活性有明显的增加,以及VIGS-NaCl和VIGS-PEG植物中MDA的显着变化。转录组分析表明，ABA生物合成基因（GhNCED1）的表达水平，信号基因（GhABI1，GhABI2和GhABI5），响应基因（GhCOR47，GhRD22和GhERFs），在干旱和NaCl处理下，VIGS品系的胁迫相关标记基因GhLEA14受到调控。总之,GhAL19作为ALTF可能通过调节抗氧化能力和ABA介导的途径负调节对干旱和盐的耐受性。
