PDF(Pubmed)
Abstract：
The wild strawberry (Fragaria vesca L.; F. vesca) represents a resilient and extensively studied model organism. While the AP2/ERF gene family plays a pivotal role in plant development, its exploration within F. vesca remains limited. In this study, we characterized the AP2/ERF gene family in wild strawberries using the recently released genomic data (F. vesca V6.0). We conducted an analysis of the gene family expansion pattern, we examined gene expression in stem segments and leaves under cold conditions, and we explored its functional attributes. Our investigation revealed that the FvAP2/ERF family comprises 86 genes distributed among four subfamilies: AP2 (17), RAV (6), ERF (62), and Soloist (1). Tandem and segmental duplications significantly contributed to the growth of this gene family. Furthermore, predictive analysis identified several cis-acting elements in the promoter region associated with meristematic tissue expression, hormone regulation, and resistance modulation. Transcriptomic analysis under cold stress unveiled diverse responses among multiple FvAP2/ERFs in stem segments and leaves. Real-time fluorescence quantitative reverse transcription PCR (RT-qPCR) results confirmed elevated expression levels of select genes following the cold treatment. Additionally, overexpression of FvERF23 in Arabidopsis enhanced cold tolerance, resulting in significantly increased fresh weight and root length compared to the wild-type control. These findings lay the foundation for further exploration into the functional roles of FvAP2/ERF genes.
摘要：
野草莓（FragariavescaL.;F.vesca）代表了一种有弹性且经过广泛研究的模型生物。而AP2/ERF基因家族在植物发育中起着举足轻重的作用,它在F.vesca内部的探索仍然有限。在这项研究中,我们使用最近发布的基因组数据表征了野草莓中的AP2/ERF基因家族(F.vescaV6.0)。我们对基因家族扩展模式进行了分析，我们在寒冷条件下检查了茎段和叶片中的基因表达，我们探索了它的功能属性。我们的调查显示，FvAP2/ERF家族包含分布在四个亚家族中的86个基因：AP2（17），RAV(6),ERF(62),和独奏家（1）。串联和分段重复显着促进了该基因家族的生长。此外，预测分析确定了与分生组织表达相关的启动子区域中的几个顺式作用元件，激素调节，和电阻调制。冷胁迫下的转录组分析揭示了茎段和叶中多个FvAP2/ERF之间的不同反应。实时荧光定量逆转录PCR(RT-qPCR)结果证实在冷处理之后选择的基因的表达水平升高。此外,FvERF23在拟南芥中的过表达增强了耐寒性，与野生型对照相比，导致鲜重和根长度显着增加。这些发现为进一步探索FvAP2/ERF基因的功能作用奠定了基础。
