多项研究表明,植物中不同营养素之间存在很强的关系,氮在锌的获取和转运中的重要作用已得到认可。
这项研究的目的是评估锌对氮吸收的影响,易位,和在水稻中的分布以及相应的分子机制。我们还旨在评估氮对稻谷中Zn含量的影响,这与稻米饮食中人类的Zn营养密切相关。
我们对两个水稻品种进行了田间试验和水培培养,以分析其生长和产量。吸收,易位,以及氮和锌的分布,以及N转运和同化基因的表达,氮和锌不同联合应用下的锌转运蛋白基因。
锌的供应促进了根至茎的转运(增加12-70%)和氮向叶片(增加19-49%)和糙米(增加6-9%)的分配,并增加了水稻生物量(14-35%)和产量(13-63%)。锌供应诱导OsNRTs和OsAMTs在根和芽中的表达,但抑制了OsNiR2,OsGS1;2和OsFd-GOGAT在根中的表达,而它激活了芽中OsNiR2,OsGS1;1,OsGS2和OsFd-GOGAT的表达。此外,亚硝酸还原酶的酶活性,硝酸还原酶,谷氨酰胺合成酶增加,游离NO3-浓度降低,但锌供应后,芽中的可溶性蛋白质浓度显着增加。协同,N显着促进了根至茎的易位(1.68-11.66倍)和Zn在叶片中的分布(1.68-6.37倍)和糙米(增加7-12%),并上调了Zn转运蛋白基因的表达水平。根和芽。
我们提出了水稻植株中锌和氮之间的串扰的工作模型,这将有助于在田间适当组合施用Zn和N肥料,以提高植物中的N利用率和以水稻为基础的饮食中的Zn营养。
Multiple studies have shown strong relationships between different nutrients in plants, and the important role of N in Zn acquisition and translocation has been recognized.
The aim of this study was to estimate the effect of Zn on N uptake, translocation, and distribution in rice as well as the corresponding molecular mechanisms. We also aimed to evaluate the impact of N on the Zn content in rice grains which is closely related to the Zn nutrition in humans with rice-based diets.
We conducted both field trials and hydroponic cultures of two rice cultivars to analyze the growth and yield, the uptake, translocation, and distribution of N and Zn, as well as the expression of N transport and assimilation genes, and the Zn transporter genes under different combined applications of N and Zn.
Zn supply promoted the root-to-shoot translocation (12-70% increasing) and distribution of N into the leaves (19-49% increasing) and brown rice (6-9% increasing) and increased the rice biomass (by 14-35%) and yield (by 13-63%). Zn supply induced the expression of OsNRTs and OsAMTs in both roots and shoots, but repressed the expression of OsNiR2, OsGS1;2, and OsFd-GOGAT in roots, whereas it activated the expression of OsNiR2, OsGS1;1, OsGS2, and OsFd-GOGAT in the shoots. Moreover, the enzyme activities of nitrite reductase, nitrate reductase, and glutamine synthetase increased and the free NO3 - concentration decreased, but the soluble protein concentration increased significantly in the shoots after Zn supply. Synergistically, N significantly facilitated the root-to-shoot translocation (1.68-11.66 fold) and distribution of Zn into the leaves (1.68-6.37 fold) and brown rice (7-12% increasing) and upregulated the expression levels of Zn transporter genes in both the roots and shoots.
We propose a working model of the cross-talk between Zn and N in rice plants, which will aid in the appropriate combined application of Zn and N fertilizers in the field to improve both N utilization in plants and Zn nutrition in humans with rice-based diets.