目的:已经开发了几种材料来保持纸浆活力。它们应具有理想的细胞相容性特征,以促进人脱落乳牙(SHED)的干细胞活性,从而治愈牙髓组织。
目的:评价不同稀释度的生物陶瓷材料提取物在SHED中的细胞毒性。
方法:根据以下实验组将SHED浸入αMEM+材料提取物中:第1组(G1)-BBio膜,第2组(G2)-Bio-C修复,组3(G3)-MTA修理HP,第4组(G4)-TheraCalLC,和第5组(G5)-生物牙本质。阳性和阴性对照组分别维持在αMEM+10%FBS和Milli-Q水中。分析细胞活力和增殖的方法涉及在SHED与生物陶瓷提取物以1:1和1:2稀释度接触后24、48和72H的MTT和AlamarBlue测定。数据通过三因素方差分析进行分析,其次是Tukey检验(p<0.05)。
结果:以1:1稀释,与MTAHP修复提取物接触的SHED比其他实验组和阴性对照显示出统计学上更高的细胞活力(p<0.05),除了TheraCalLC(p>0.05)。在1:2稀释时,BBio膜和Bio-C在组内和组间比较中显示出统计学上更高的值(p<0.05)。BBio膜,Bio-C修复,在所有时期,1:1稀释的生物牙本质提取物显示比1:2稀释更大的细胞毒性(p<0.05)。
结论:MTAHP修复即使在1:1稀释时也显示出最低的细胞毒性。以1:2的稀释度,与BBio膜提取物接触的SHED显示高细胞活力。因此,Bio膜将是一种新型的非细胞毒性生物材料。结果提供了生物材料的可能性,可用于牙本质-牙髓复合物的临床再生程序。
OBJECTIVE: Several materials have been developed to preserve pulp vitality. They should have ideal cytocompatibility characteristics to promote the activity of stem cells of human exfoliated deciduous teeth (SHED) and thus heal pulp tissue.
OBJECTIVE: To evaluate the cytotoxicity of different dilutions of bioceramic material extracts in SHED.
METHODS: SHED were immersed in αMEM + the material extract according to the following experimental groups: Group 1 (G1) -BBio membrane, Group 2 (G2) - Bio-C Repair, Group 3 (G3) - MTA Repair HP, Group 4 (G4) - TheraCal LC, and Group 5 (G5) - Biodentine. Positive and negative control groups were maintained respectively in αMEM + 10% FBS and Milli-Q Water. The methods to analyze cell viability and proliferation involved MTT and Alamar Blue assays at 24, 48, and 72H after the contact of the SHED with bioceramic extracts at 1:1 and 1:2 dilutions. Data were analyzed by the three-way ANOVA, followed by Tukey\'s test (p<0.05).
RESULTS: At 1:1 dilution, SHED in contact with the MTA HP Repair extract showed statistically higher cell viability than the other experimental groups and the negative control (p<0.05), except for TheraCal LC (p> 0.05). At 1:2 dilution, BBio Membrane and Bio-C showed statistically higher values in intra- and intergroup comparisons (p<0.05). BBio Membrane, Bio-C Repair, and Biodentine extracts at 1:1 dilution showed greater cytotoxicity than 1:2 dilution in all periods (p<0.05).
CONCLUSIONS: MTA HP Repair showed the lowest cytotoxicity even at a 1:1 dilution. At a 1:2 dilution, the SHED in contact with the BBio membrane extract showed high cell viability. Thus, the BBio membrane would be a new non-cytotoxic biomaterial for SHED. Results offer possibilities of biomaterials that can be indicated for use in clinical regenerative procedures of the dentin-pulp complex.