{Reference Type}: Journal Article
{Title}: Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling.
{Author}: Chang Y;Yi JY;Brotto M;Pan Z;
{Journal}: Methods Mol Biol
{Volume}: 2816
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3902-3_7
{Abstract}: Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we describe the protocols to measure intracellular Ca2+ for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.