%0 Journal Article
%T Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling.
%A Chang Y
%A Yi JY
%A Brotto M
%A Pan Z
%J Methods Mol Biol
%V 2816
%N 0
%D 2024
%M 38977589
暂无%R 10.1007/978-1-0716-3902-3_7
%X Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we describe the protocols to measure intracellular Ca2+ for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.