Abstract：
Intracellular Ca2+ can be conveniently monitored by sensitive Ca2+ fluorescent dyes in live cells. The Gαq involved lipid signaling pathways and, thus, can be studied by intracellular Ca2+ imaging. Here we describe the protocols to measure intracellular Ca2+ for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.
摘要：
通过活细胞中敏感的Ca2荧光染料可以方便地监测细胞内Ca2。Gαq涉及脂质信号通路，因此，可以通过细胞内Ca2+成像来研究。在这里，我们描述了测量细胞内Ca2+以研究食管平滑肌细胞中PEG2-EP1活性的方案。比率Fura-2成像提供了定量数据，Fluo-4共聚焦显微成像具有较高的空间分辨率。
