细胞内 Ca2 + 的测量用于研究 GPCR 介导的脂质信号传导。
关键词: Ca2+ signaling Esophageal smooth muscle Fluo-4 AM Fura-2 AM G-protein-coupled receptor Lipid signaling mediator PGE-2 Ratiometric dye Second messenger
Mesh : Calcium / metabolism Receptors, G-Protein-Coupled / metabolism Animals Microscopy, Confocal / methods Signal Transduction Myocytes, Smooth Muscle / metabolism Calcium Signaling Humans Xanthenes / metabolism Fura-2 / metabolism Lipid Metabolism Esophagus / metabolism Fluorescent Dyes / chemistry metabolism Aniline Compounds
来 源: DOI:10.1007/978-1-0716-3902-3_7