目的:我们旨在从放射学上确定香草酸(VA)对骨折愈合的影响,组织学上,免疫组织化学,并采用生物力学建立大鼠股骨开放性骨折损伤模型。
方法:32只雄性Wistar-Albino大鼠,分为两组:研究组(VA)和对照组。从他们做手术到牺牲,研究组大鼠经口灌胃给予100mg/kg/dVA。牺牲后,对股骨进行了分析。
结果:观察到VA组的Huo组织学评分明显更高(p=0.001),骨痂组织的数量与完整骨组织的比例明显更高。虽然在ColI抗体染色的免疫组织化学H评分中没有观察到显著差异(p=1.000),在ColIII抗体染色中观察到有利于VA的边界显着差异(p=0.078)。在生物力学分析中,失效载荷(N),总能量(J),最大应力(MPa),VA组的硬度(N/mm)测量值显著高于VA组(分别为p=0.040,p=0.021,p=0.015和p=0.035).
结论:已经观察到VA,凭借其抗氧化性能,增加大鼠的骨折愈合,其中创建了一个开放性骨折模型。我们希望这种抗氧化剂,这在自然界中很常见,会增加骨折愈合。由于这项研究是第一个检查VA对骨折愈合的影响,需要进一步的研究。
OBJECTIVE: We aimed to determine the effects of vanillic acid (VA) on fracture healing radiologically, histologically, immunohistochemically, and biomechanically using a rat femur open fracture injury model.
METHODS: 32 male Wistar-Albino rats were used and divided into two groups: the study group (VA) and the control group. From the time they were operated on until they were sacrificed, the rats in the study group were given 100 mg/kg/day VA by oral gavage. After sacrification, the femurs were analyzed.
RESULTS: It was observed that the Huo histological scoring was significantly higher in the VA group (p = 0.001), and the ratio of the amount of callus tissue compared to intact bone tissue was significantly higher. While no significant difference was observed in immunohistochemical H-scores in ColI antibody staining (p = 1.000), a borderline significant difference in favor of VA was observed in ColIII antibody staining (p = 0.078). In biomechanical analysis, failure load (N), total energy (J), maximum stress (MPa), and stiffness (N/mm) measurements were significantly higher in the VA group (p = 0.040, p = 0.021, p = 0.015, and p = 0.035, respectively).
CONCLUSIONS: It has been observed that VA, with its antioxidative properties, increases fracture healing in rats, in which an open fracture model was created. We are hopeful that such an antioxidant, which is common in nature, will increase fracture healing. Since this study is the first to examine the effect of VA on fracture healing, further studies are needed.