PDF(Pubmed)
Abstract：
Recently, nanoparticles have received considerable attention owing to their efficiency in overcoming the limitations of traditional chemotherapeutic drugs. In our study, we synthesized a vanillic acid nanocomposite using both chitosan and silver nanoparticles, tested its efficacy against lung cancer cells, and analyzed its antimicrobial effects. We used several characterization techniques such as ultraviolet-visible spectroscopy (UV-Vis), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDAX), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) to determine the stability, morphological characteristics, and properties of the biosynthesized vanillic acid nanocomposites. Furthermore, the vanillic acid nanocomposites were tested for their antimicrobial effects against Escherichia coli and Staphylococcus aureus, and Candida albicans. The data showed that the nanocomposite effectively inhibited microbes, but its efficacy was less than that of the individual silver and chitosan nanoparticles. Moreover, the vanillic acid nanocomposite exhibited anticancer effects by increasing the expression of pro-apoptotic proteins (BAX, Casp3, Casp7, cyt C, and p53) and decreasing the gene expression of Bcl-2. Overall, vanillic acid nanocomposites possess promising potential against microbes, exhibit anticancer effects, and can be effectively used for treating diseases such as cancers and infectious diseases.
摘要：
最近,纳米颗粒由于其在克服传统化疗药物的局限性方面的效率而受到了相当大的关注。在我们的研究中,我们使用壳聚糖和银纳米颗粒合成了香草酸纳米复合材料，测试了它对肺癌细胞的功效，并分析了其抗菌效果。我们使用了几种表征技术，如紫外-可见光谱(UV-Vis)，场发射扫描电子显微镜（FESEM），能量色散X射线光谱（EDAX），热重分析（TGA），和差示扫描量热法（DSC）来确定稳定性，形态特征，生物合成香草酸纳米复合材料的性能。此外，测试了香草酸纳米复合材料对大肠杆菌和金黄色葡萄球菌的抗菌作用，和白色念珠菌.数据表明，纳米复合材料有效地抑制了微生物，但其功效低于单独的银和壳聚糖纳米颗粒。此外，香草酸纳米复合材料通过增加促凋亡蛋白的表达而表现出抗癌作用(BAX，Casp3,Casp7,cytC,和p53）并降低Bcl-2的基因表达。总的来说,香草酸纳米复合材料具有对微生物有前途的潜力，表现出抗癌作用，可有效用于治疗癌症和传染病等疾病。
