Abstract:
The engineered nano-vehicle was constructed using magnetic iron oxide nanoparticles (MIONs) and chitosan (CTS) to stabilize anticancer agent vanillic acid (VNA) which was loaded on CTS-coated MIONs nanocarrier, and more importantly, to achieve sustained VNA release and subsequent proper anticancer activity. The new thermally stable VNA-CTS- MIONs nanocomposite was spherical with a middle diameter of 6 nm and had a high drug loading of about 11.8 %. The MIONs and resulting nanocomposite were composed of pure magnetite and therefore, were superparamagnetic with saturation magnetizations of 53.3 and 45.7 emu.g-1, respectively. The release profiles of VNA from VNA-CTS-MIONs nanocomposite in different pH values were sustained and showed controlled pH-responsive delivery of the loaded VNA with 89 % and 74 % percentage release within 2354 and 4046 min at pH 5 and 7.4, respectively, as well as were in accordance with the pseudo-second-order model. The VNA-CTS-MIONs nanocomposite treatment at diverse concentrations remarkably decreased the viability and promoted ROS accumulation and apoptosis in the MDA-MB-231 breast cancer cells. Hence, it can be a propitious candidate for the management of breast cancer in the future.
摘要:
使用磁性氧化铁纳米颗粒(MIONS)和壳聚糖(CTS)构建工程纳米载体,以稳定负载在CTS包覆的MIONS纳米载体上的抗癌剂香草酸(VNA),更重要的是,以实现持续的VNA释放和随后的适当抗癌活性。新的热稳定的VNA-CT-MIONs纳米复合材料为球形,中间直径为6nm,并且具有约11.8%的高载药量。MION和所得的纳米复合材料由纯磁铁矿组成,因此,是超顺磁性的,饱和磁化强度为53.3和45.7emu。分别为g-1。在不同pH值下VNA-CTS-MIONs纳米复合材料中VNA的释放曲线是持续的,并显示出负载的VNA的受控pH响应性递送,在pH5和7.4分别在2354和4046分钟内释放89%和74%百分比,以及符合伪二阶模型。不同浓度的VNA-CTS-MIONs纳米复合材料处理显著降低MDA-MB-231乳腺癌细胞的活力并促进ROS积累和凋亡。因此,它可能是未来乳腺癌治疗的有利人选。