Abstract：
Caldimonas thermodepolymerans, a Gram-negative, moderately thermophilic bacterium, exhibits a remarkable biotechnological potential. Given the presence of genes in its genome dedicated to the metabolization of ferulic acid (FA), this study aimed to explore the bacterium\'s capability for biotransforming FA into high-value metabolites. The results unequivocally demonstrate the bacterium\'s proficiency in the efficient and rapid conversion of FA into vanillyl alcohol (VOH) and vanillic acid (VA). By manipulating key cultivation parameters, such as adjusting initial FA doses and varying cultivation periods, the product profile can be tailored. Higher initial doses and shorter cultivation periods favor the production of VOH, while lower FA doses and extended cultivation periods lead to the predominant formation of VA. Furthermore, the process can be operated in a repeated-batch scenario. This underscores the potential of C. thermodepolymerans for industrial biotransformation of FA, presenting a promising avenue for leveraging its capabilities in practical applications.
摘要：
Caldimonas热解聚物，革兰氏阴性，中度嗜热细菌，具有显著的生物技术潜力。鉴于其基因组中存在致力于阿魏酸（FA）代谢的基因，本研究旨在探讨细菌将FA生物转化为高价值代谢产物的能力。结果明确地证明了该细菌在将FA有效和快速地转化为香草醇（VOH）和香草酸（VA）方面的熟练程度。通过操纵关键栽培参数，如调整初始FA剂量和不同的培养期，产品简介可以定制。较高的初始剂量和较短的培养期有利于VOH的生产，而较低的FA剂量和延长的培养期导致VA的主要形成。此外，该过程可以在重复分批的情况下操作。这强调了C.thermodelpolyans用于FA的工业生物转化的潜力，为在实际应用中利用其能力提供了一条有希望的途径。
