基于蛋白质的纳米颗粒(PNP)在肿瘤治疗中具有巨大的潜力,结合有针对性的交付,最小的毒性,和可自定义的属性,从而为癌症治疗的创新方法铺平了道路。了解可用于生产的各种方法对于旨在利用这些纳米颗粒进行各种应用的研究人员和科学家至关重要。包括肿瘤治疗,药物输送,成像,和组织工程。这篇综述深入研究了生产PNP和PNP/药物复合物的现有技术,同时也在探索替代的新方法。本研究中概述的方法根据其共同的程序步骤分为三个关键类别:溶解度变化,溶剂取代,和稀流方法。这种分类通过提供一个明确的框架来简化对底层机制的理解,提供了优于其他分类的几个优点。这篇综述讨论了每种方法的基本原理,突出了影响纳米颗粒尺寸的因素,形态学,稳定性,和功能。它还解决了与每种方法相关的挑战和注意事项,包括可扩展性,再现性,和生物相容性。讨论了PNP生产的未来前景和新兴趋势,强调创新战略克服当前局限性的潜力,这将推动生物医学和治疗应用领域向前发展。
Protein-based nanoparticles (PNPs) in tumor therapy hold immense potential, combining targeted delivery, minimal toxicity, and customizable properties, thus paving the way for innovative approaches to cancer treatment. Understanding the various methods available for their production is crucial for researchers and scientists aiming to harness these nanoparticles for diverse applications, including tumor therapy, drug delivery, imaging, and tissue engineering. This review delves into the existing techniques for producing PNPs and PNP/drug complexes, while also exploring alternative novel approaches. The methods outlined in this study were divided into three key categories based on their shared procedural steps: solubility change, solvent substitution, and thin flow methods. This classification simplifies the understanding of the underlying mechanisms by offering a clear framework, providing several advantages over other categorizations. The review discusses the principles underlying each method, highlighting the factors influencing the nanoparticle size, morphology, stability, and functionality. It also addresses the challenges and considerations associated with each method, including the scalability, reproducibility, and biocompatibility. Future perspectives and emerging trends in PNPs\' production are discussed, emphasizing the potential for innovative strategies to overcome current limitations, which will propel the field forward for biomedical and therapeutic applications.