■LIN9,一种与各种癌症相关的基因,被认为是肿瘤抑制剂。然而,LIN9在肺腺癌(LUAD)中的作用尚不清楚.在这项研究中,我们旨在评估LIN9在LUAD发生和预后中的作用.
■使用来自癌症基因组图谱的三层HTSeq计数RNA测序数据,我们使用DESeq2R包和RT-qPCR实验评估了LUAD数据集的LIN9表达。使用基因集富集分析(clusterProfiler和GOplot)评估生物学功能。通过单样品基因集富集分析评估LIN9的表达和免疫细胞的浸润。我们使用临床特征和受试者工作特征曲线分析进行了相关性研究。LIN9的预测值使用单变量和多变量Cox回归以及Kaplan-Meier分析确定。此外,进行了功能研究以验证其在LUAD进展中的作用.
■LIN9在LUAD中的表达显著升高,主要影响细胞周期,司,和信号通路。LIN9的高表达与Th2细胞的浸润呈正相关,与浆细胞样树突状细胞的浸润呈负相关。此外,LIN9与年龄较大和临床分期晚期有关,对整体构成风险,无进展,和疾病特异性生存。LIN9是一个很好的诊断标记,尤其是女性,65岁以上的患者,以及临床N1-3和M1期的患者。升高的LIN9表达增强了增殖,迁移,和LUAD细胞的侵袭。
■高LIN9表达可能通过细胞周期调控和染色体修饰促进LUAD的发生。它促进LUAD细胞的恶性特征,并对受影响的患者具有预后价值。
UNASSIGNED: LIN9, a gene associated with various cancers, is considered a tumor suppressor. However, the role of LIN9 in lung adenocarcinoma (LUAD) remains unknown. In this study, we aimed to assess the role of LIN9 in the occurrence and prognosis of LUAD.
UNASSIGNED: Using three-tier HTSeq count RNA sequencing data from The Cancer Genome Atlas, we assessed LIN9 expression for the LUAD dataset using the DESeq2 R package and RT-qPCR experiments. Biological functions were assessed using gene set enrichment analysis (clusterProfiler and GOplot). The expression of LIN9 and the infiltration of immune cells were assessed by Single-sample gene set enrichment analysis. We conducted correlation study using clinical characteristics and receiver operating characteristic curve analysis. The predictive value of LIN9 was determined using univariate and multivariate Cox regression as well as Kaplan-Meier analysis. Additionally, functional studies were conducted to validate its role in the progression of LUAD.
UNASSIGNED: Expression of LIN9 was significantly elevated in LUAD, primarily influencing cell cycle, division, and signaling pathways. High LIN9 expression correlated positively with the infiltration of Th2 cells and inversely with that of plasmacytoid dendritic cells. Furthermore, LIN9 was associated with older age and advanced clinical stages, posing risks to overall, progression-free, and disease-specific survival. LIN9 served as a good diagnostic marker, particularly in females, patients aged over 65, and those with clinical N1-3 and M1 stages. Elevated LIN9 expression enhanced proliferation, migration, and invasion of LUAD cells.
UNASSIGNED: High LIN9 expression potentially contributes to LUAD occurrence through cell cycle regulation and chromosomal modification. It promotes the malignant characteristics of LUAD cells and holds prognostic value for affected patients.