在真菌中,MYB转录因子(TFs)主要调节生长,发展,和抵抗压力。然而,作为主要的抗病TFs,它们很少在生物防治真菌中进行研究。在这项研究中,木霉Tas653(Ta)的MYB36被证明对链格孢菌Aa1004引起的胁迫有强烈的反应。与野生型Ta(Ta-Wt)相比,MYB36敲除菌株(Ta-Kn)对Aa1004的抑制率下降11.06%;超氧化物歧化酶(SOD),过氧化物酶(POD),过氧化氢酶(CAT)活性降低82.15U/g,0.19OD470/min/g,和1631.2μmol/min/g,分别。MYB36过表达菌株(Ta-Oe)不仅增强了Aa1004的高寄生作用,还导致其菌丝膨胀,变形,甚至破裂,同时也降低了杨树叶枯病的发病率。MYB36调节下游(TFs,解毒基因,防御基因,和其他抗真菌相关基因通过与顺式作用元件“ACAT”和“ATCG”结合。锌指TFs,作为主要的抗真菌TFs,占总TFs的90%,当直接受MYB36调节时,Zn37.5(23.24-)和Zn83.7(23.18倍)显示出最大的表达差异。解毒基因主要包括11个主要的主要促进因子超家族(MFS)基因,其中MYB36直接使三个基因的表达水平提高了2-3.44倍以上。防御基因主要编码细胞色素P450(P450)和水解酶。例如,P45061.3(2-10.95-),P45060.2(2-7.07-),和Hyd44.6(2-2.30倍)。本研究揭示了MYB36调控杨树叶枯病抗性的分子机制,为杨树叶枯病的生物防治和生防真菌的抗病机理研究提供了理论指导。
In fungi, MYB transcription factors (TFs) mainly regulate growth, development, and resistance to stress. However, as major disease-resistance TFs, they have rarely been studied in biocontrol fungi. In this study, MYB36 of Trichoderma asperellum Tas653 (Ta) was shown to respond strongly to the stress caused by Alternaria alternata Aa1004. Compared with wild-type Ta (Ta-Wt), the inhibition rate of the MYB36 knockout strain (Ta-Kn) on Aa1004 decreased by 11.06%; the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities decreased by 82.15 U/g, 0.19 OD470/min/g, and 1631.2 μmol/min/g, respectively. The MYB36 overexpression strain (Ta-Oe) not only enhanced hyperparasitism on Aa1004, caused its hyphae to swell, deform, or even rupture, but also reduced the incidence rate of poplar leaf blight. MYB36 regulates downstream (TFs, detoxification genes, defense genes, and other antifungal-related genes by binding to the cis-acting elements \"ACAT\" and \"ATCG\". Zinc finger TFs, as the main antifungal TFs, account for 90% of the total TFs, and Zn37.5 (23.24-) and Zn83.7 (23.18-fold) showed the greatest expression difference when regulated directly by MYB36. The detoxification genes mainly comprised 11 major major facilitator superfamily (MFS) genes, among which MYB36 directly increased the expression levels of three genes by more than 2-3.44-fold. The defense genes mainly encoded cytochrome P450 (P450) and hydrolases. e.g., P45061.3 (2-10.95-), P45060.2 (2-7.07-), and Hyd44.6 (2-2.30-fold). This study revealed the molecular mechanism of MYB36 regulation of the resistance of T. asperellum to A. alternata and provides theoretical guidance for the biocontrol of poplar leaf blight and the anti-disease mechanism of biocontrol fungi.