PDF(Pubmed)
Abstract：
Stem-end rot disease has been causing damage to the production of pomelos in Vietnam. The cur-rent study aimed to (i) isolate fungal pathogens causing pomelo stem-end rot disease (PSERD) and (ii) discover Trichoderma spp. that had an antagonistic ability against pathogens under in vitro conditions. Fungi causing PSERD were isolated from pomelo fruits with symptoms of stem-end rot disease and collected from pomelo farms in Ben Tre province, Vietnam. Moreover, 50 fungal strains of Trichoderma spp. also originated from soils of these pomelo farms in Ben Tre province and were dual-tested with the fungal pathogen on the PDA medium. The results demonstrated that 11 pathogenic fungi causing PSERD were isolated from the fruit and showed mycelial growth of roughly 5.33-8.77 cm diameter at 72 h after inoculation. The two fungi that exhibited the fast-est growth, namely, S-P06 and S-P07, were selected. ITS sequencing of the S-P06 and S-P07 fungi resulted in Lasiodiplodia theobromae. All the 50 Trichoderma spp. strains were allowed to antago-nize against the S-P06 and S-P07 strains under in vitro conditions. The greatest antagonistic effi-ciency was found in Trichoderma spp. T-SP19 at 85.4-86.2% and T-SP32 at 84.7-85.4%. The two antagonists were identified as Trichoderma asperellum T-SP19 and T-SP32. The selected strains of Trichoderma asperellum were potent as a biological control for fruit plants.
摘要：
茎端腐烂病一直对越南的柚子生产造成损害。目前的研究旨在（i）分离引起柚子茎端腐烂病（PSERD）的真菌病原体和（ii）发现木霉属。在体外条件下对病原体具有拮抗能力。从具有茎端腐烂病症状的柚子果实中分离出引起PSERD的真菌，并从本特雷省的柚子农场收集，越南。此外，木霉属真菌50株。也起源于本特雷省这些柚子农场的土壤，并在PDA培养基上用真菌病原体进行了双重测试。结果表明，从果实中分离出11种引起PSERD的病原真菌，接种后72小时，菌丝生长约为5.33-8.77cm。表现出最快生长的两种真菌，即,选择S-P06和S-P07。S-P06和S-P07真菌的ITS测序导致Lasiodiplodiatheobromae。所有50种木霉属。在体外条件下，使菌株抗S-P06和S-P07菌株。在木霉属中发现了最大的拮抗效力。T-SP19为85.4-86.2%，T-SP32为84.7-85.4%。这两种拮抗剂被鉴定为天冬木霉T-SP19和T-SP32。选定的木霉菌菌株可有效用作水果植物的生物防治。
