关键词: 6-pentyl-alpha-pyrone Trichoderma asperellum experimental design lytic enzymes solid-state fermentation spores

来  源:   DOI:10.3390/jof9111123   PDF(Pubmed)

Abstract:
Managing organic agricultural wastes is a challenge in today\'s modern agriculture, where the production of different agricultural goods leads to the generation of large amounts of waste, for example, olive pomace and vine shoot in Mediterranean Europe. The discovery of a cost-effective and environment-friendly way to valorize such types of waste in Mediterranean Europe is encouraged by the European Union regulation. As an opportunity, organic agricultural waste could be used as culture media for solid-state fermentation (SSF) for fungal strains. This methodology represents a great opportunity to produce secondary metabolites like 6-pentyl-alpha-pyrone (6-PP), a lactone compound with antifungal properties against phytopathogens, produced by Trichoderma spp. Therefore, to reach adequate yields of 6-PP, lytic enzymes, and spores, optimization using specific agricultural cheap local wastes from Southeastern France is in order. The present study was designed to show the applicability of an experimental admixture design to find the optimal formulation that favors the production of 6-PP. To conclude, the optimized formulation of 6-PP production by Trichoderma under SSF contains 18% wheat bran, 23% potato flakes, 20% olive pomace, 14% olive oil, 24% oatmeal, and 40% vine shoots.
摘要:
管理有机农业废物是当今现代农业的挑战,不同农产品的生产导致大量废物的产生,例如,欧洲地中海的橄榄果渣和藤本植物。欧盟法规鼓励在地中海欧洲发现一种具有成本效益和环境友好的方式来评估此类废物的价值。作为一个机会,有机农业废物可用作真菌菌株固态发酵(SSF)的培养基。这种方法代表了一个很好的机会,产生次级代谢产物,如6-戊基-α-吡喃酮(6-PP),对植物病原体具有抗真菌特性的内酯化合物,木霉属。因此,为了达到6-PP的足够产率,裂解酶,和孢子,使用法国东南部特定的农业廉价当地废物进行优化。本研究旨在显示实验混合物设计的适用性,以找到有利于生产6-PP的最佳配方。最后,SSF下木霉生产6-PP的优化配方含有18%的麦麸,23%的马铃薯片,20%橄榄果渣,14%橄榄油,24%的燕麦片,和40%的葡萄树芽。
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