背景:成骨细胞反应在口腔植入物的成功中起着至关重要的作用。成骨细胞增殖增强与细胞死亡率降低和骨再生增加相关。本研究旨在评估口腔植入后的成骨细胞反应。
方法:收获成骨细胞干细胞,随后使用细胞培养技术进行培养。通过检测细胞外基质确认提取细胞的成骨细胞表型。通过间接免疫荧光染色评估功能化生物材料表面上的细胞形态发生。在两种类型的植入物材料存在下研究了细胞反应:钛(Ti)和氧化铝增韧的氧化锆(ATZ)。使用MTT测定和流式细胞术定量评估细胞活力和凋亡,分别。
结果:植入后成骨细胞谱系细胞的存活率中度降低。Ti植入物组的生存力保持在约86%,而在ATZ组,它被观察到75%,这被认为是可以接受的。此外,两个植入物组之间的细胞存活率存在显着差异(p<0.05)。不同浓度的细胞凋亡水平分析显示,对照组细胞凋亡率为3.6%,ATZ组细胞凋亡率为18.5%,表明ATZ处理组的细胞凋亡或程序性细胞死亡增加了近4倍(p<0.05)。
结论:这项研究的结果表明植入治疗后成骨细胞的存活率降低,钛植入物在细胞存活方面表现出优异的性能。然而,还注意到,在锆基植入物存在的情况下,成骨细胞凋亡的发生率显著较高.
BACKGROUND: Osteoblastic responses play a crucial role in the success of oral implants. Enhanced proliferation of osteoblast cells is associated with reduced cell mortality and an increase in bone regeneration. This study aims to evaluate the osteoblastic responses following oral implantation.
METHODS: Osteoblast stem cells were harvested and subsequently cultivated using cell culture techniques. The osteoblastic phenotype of the extracted cells was confirmed by examining the extracellular matrix. Cell morphogenesis on functionalized biomaterial surfaces was assessed through indirect immunofluorescence staining. The cellular response was investigated in the presence of two types of implant materials: titanium (Ti) and alumina-toughened zirconia (ATZ). Cell viability and apoptosis were quantitatively assessed using MTT assays and flow cytometry, respectively.
RESULTS: The survival of osteoblastic lineage cells was moderately reduced post-implantation. Viability in the Ti implant group remained at approximately 86%, while in the ATZ group, it was observed at 75%, which is considered acceptable. Moreover, there was a significant disparity in cell survival between the two implant groups (p < 0.05). Analysis of apoptosis levels at various concentrations revealed that the rate of apoptosis was 3.6% in the control group and 18.5% in the ATZ group, indicating that apoptosis or programmed cell death in the ATZ-treated group had increased nearly four-fold (p < 0.05).
CONCLUSIONS: The findings of this study indicate a reduction in osteoblastic cell line survival following implant treatment, with titanium implants exhibiting superior performance in terms of cell survival. However, it was also noted that the incidence of apoptosis in osteoblast cells was significantly higher in the presence of zirconium-based implants.