PDF(Pubmed)
Abstract：
BACKGROUND: The search for effective antimicrobial agents to mitigate peri-implant infections remains a crucial aspect of implant dentistry. This study aimed to evaluate and compare the antimicrobial efficacy of i-PRF, A-PRF+, and enamel matrix derivative (EMD) on decontaminated rough and smooth titanium (Ti) discs.
METHODS: Rough and smooth Ti discs were coated with multispecies biofilm and thoroughly debrided using a chitosan-bristled brush. Subsequently, i-PRF, A-PRF+, and EMD were applied. Untreated discs served as control. Residual adherent bacteria present on the treated Ti discs were visualized by SEM and quantified using culture technique, and colony-forming units (CFUs) were measured after 48 h and 7 days.
RESULTS: i-PRF demonstrated better antimicrobial effectiveness on both smooth and rough implant surfaces as compared to A-PRF+ and EMD (p < 0.001). In all the experimental groups, smooth Ti discs displayed a greater reduction in microbes compared to rough Ti discs when treated with the biologics. The major reduction in CFU values was determined after seven days.
CONCLUSIONS: i-PRF as a regenerative material may also be suitable for decontaminating implant surfaces, which could influence tissue healing and regenerative outcomes positively.
METHODS: Rough and smooth Ti discs were coated with multispecies biofilm and thoroughly debrided using a chitosan-bristled brush. Subsequently, i-PRF, A-PRF+, and EMD were applied. Untreated discs served as control. Residual adherent bacteria present on the treated Ti discs were visualized by SEM and quantified using culture technique, and colony-forming units (CFUs) were measured after 48 h and 7 days.
RESULTS: i-PRF demonstrated better antimicrobial effectiveness on both smooth and rough implant surfaces as compared to A-PRF+ and EMD (p < 0.001). In all the experimental groups, smooth Ti discs displayed a greater reduction in microbes compared to rough Ti discs when treated with the biologics. The major reduction in CFU values was determined after seven days.
CONCLUSIONS: i-PRF as a regenerative material may also be suitable for decontaminating implant surfaces, which could influence tissue healing and regenerative outcomes positively.
摘要：
背景：寻找有效的抗微生物剂以减轻植入物周围的感染仍然是植入物牙科的关键方面。本研究旨在评估和比较i-PRF的抗菌效果，A-PRF+,和釉质基质衍生物（EMD）在去污的粗糙和光滑的钛（Ti）盘。
方法：用多物种生物膜涂覆粗糙且光滑的Ti圆盘，并使用壳聚糖刷毛刷进行彻底清创。随后，i-PRF,A-PRF+,并应用了EMD。未处理的圆盘用作对照。通过SEM可视化处理过的Ti圆盘上存在的残留粘附细菌，并使用培养技术进行定量。在48小时和7天后测量菌落形成单位(CFU)。
结果:与A-PRF+和EMD相比,i-PRF在光滑和粗糙的植入物表面上显示出更好的抗微生物效力(p<0.001)。在所有的实验组中,与用生物制剂处理的粗糙Ti圆盘相比，光滑Ti圆盘显示出更大的微生物减少。在7天后确定CFU值的主要降低。
结论：i-PRF作为再生材料也可能适用于植入物表面的净化，这可能会积极影响组织愈合和再生结果。
方法：用多物种生物膜涂覆粗糙且光滑的Ti圆盘，并使用壳聚糖刷毛刷进行彻底清创。随后，i-PRF,A-PRF+,并应用了EMD。未处理的圆盘用作对照。通过SEM可视化处理过的Ti圆盘上存在的残留粘附细菌，并使用培养技术进行定量。在48小时和7天后测量菌落形成单位(CFU)。
结果:与A-PRF+和EMD相比,i-PRF在光滑和粗糙的植入物表面上显示出更好的抗微生物效力(p<0.001)。在所有的实验组中,与用生物制剂处理的粗糙Ti圆盘相比，光滑Ti圆盘显示出更大的微生物减少。在7天后确定CFU值的主要降低。
结论：i-PRF作为再生材料也可能适用于植入物表面的净化，这可能会积极影响组织愈合和再生结果。
