Abstract：
Dental Implants are expected to possess both excellent osteointegration and antibacterial activity because poor osseointegration and infection are two major causes of titanium implant failure. In this study, we constructed layer-by-layer self-assembly films consisting of anionic casein phosphopeptides-amorphous calcium phosphate (CPP-ACP) and cationic poly (L-lysine) (PLL) on sandblasted and acid etched (SLA) titanium surfaces and evaluated their osseointegration and antibacterial performance in vitro and in vivo. The surface properties were examined, including microstructure, elemental composition, wettability, and Ca2+ ion release. The impact the surfaces had on the adhesion, proliferation and differentiation abilities of MC3T3-E1 cells were investigated, as well as the material\'s antibacterial performance after exposure to the oral microorganisms such as Porphyromonas gingivalis (P. g) and Actinobacillus actinomycetemcomitans (A. a). For the in vivo studies, SLA and Ti (PLL/CA-3.0)10 implants were inserted into the extraction socket immediately after extracting the rabbit mandibular anterior teeth with or without exposure to mixed bacteria solution (P. g & A. a). Three rabbits in each group were sacrificed to collect samples at 2, 4, and 6 weeks of post-implantation, respectively. Radiographic and histomorphometry examinations were performed to evaluate the implant osseointegration. The modified titanium surfaces were successfully prepared and appeared as a compact nano-structure with high hydrophilicity. In particular, the Ti (PLL/CA-3.0)10 surface was able to continuously release Ca2+ ions. From the in vitro and in vivo studies, the modified titanium surfaces expressed enhanced osteogenic and antibacterial properties. Hence, the PLL/CPP-ACP multilayer coating on titanium surfaces was constructed via a layer-by-layer self-assembly technology, possibly improving the biofunctionalization of Ti-based dental implants.
摘要：
预期牙科植入物具有优异的骨整合和抗菌活性,因为不良的骨整合和感染是钛植入物失败的两个主要原因。在这项研究中,我们在喷砂和酸蚀（SLA）钛表面上构建了由阴离子酪蛋白磷酸肽-无定形磷酸钙（CPP-ACP）和阳离子聚（L-赖氨酸）（PLL）组成的逐层自组装膜，并评估了它们在体外和体内的骨整合和抗菌性能。检查了表面特性，包括微观结构，元素组成,润湿性,和Ca2+离子释放。表面对附着力的影响，研究了MC3T3-E1细胞的增殖和分化能力，以及该材料在暴露于口腔微生物如牙龈卟啉单胞菌(P.g）和放线菌放线杆菌（A.a).对于体内研究，SLA和Ti（PLL/CA-3.0）10植入物在拔除兔下颌前牙后立即插入拔牙槽中，暴露或不暴露于混合细菌溶液（P。g&A.a)。在植入后第2、4和6周，每组处死3只兔子以收集样本，分别。进行放射学和组织形态计量学检查以评估植入物的骨整合。成功制备了改性的钛表面，并表现为具有高亲水性的致密纳米结构。特别是,Ti(PLL/CA-3.0)10表面能够连续释放Ca2+离子。从体外和体内研究来看，改性后的钛表面表现出增强的成骨和抗菌性能。因此，通过逐层自组装技术在钛表面上构造PLL/CPP-ACP多层涂层，有可能改善钛基牙种植体的生物功能化。
