目的:纤维肌痛综合征(FMS)是一种病理生理未知的疾病,诊断是基于一组临床标准。蛋白质组学分析可以为疾病的病理生理学提供重要的生物学信息,但也可以揭示用于诊断或治疗靶标的生物标志物。本系统综述旨在使用观察性研究的数据综合有关FMS成年患者蛋白质组的证据。
结果:在MEDLINE/PubMed,中部,和clinicaltrials.gov从成立到2022年11月。该研究方案发表在OSF中。两名独立的审查人员评估了研究并提取了数据。使用针对蛋白质组学研究进行调整的改良的纽卡斯尔-渥太华量表评估研究质量。十项研究符合协议标准,鉴定3328种蛋白质,其中145个在FMS患者中差异表达。在血浆中鉴定出蛋白质,血清,脑脊液,还有唾液样本.对照组包括健康个体和疼痛患者(炎性和非炎性)。确定的最重要的蛋白质涉及转铁蛋白,α-,β-,和γ-纤维蛋白原链,profilin-1,转醛缩酶,PGAM1,载脂蛋白C3,补体C4A和C1QC,免疫球蛋白部分,和急性期反应物。蛋白质和痛觉之间的相关性较弱,或生活质量量表,除了转铁蛋白和α2-巨球蛋白与中度至重度疼痛的关联。纳入研究的质量为中等至良好。FMS似乎与补体和凝血级联反应中的蛋白质失调以及铁的代谢有关。由于过度的氧化应激反应,几种蛋白质可能失调。
OBJECTIVE: Fibromyalgia syndrome (FMS) is a disease of unknown pathophysiology, with the diagnosis being based on a set of clinical criteria. Proteomic analysis can provide significant biological information for the pathophysiology of the disease but may also reveal biomarkers for diagnosis or therapeutic targets. The present systematic review aims to synthesize the evidence regarding the proteome of adult patients with FMS using data from observational studies.
RESULTS: An extensive literature search was conducted in MEDLINE/PubMed, CENTRAL, and clinicaltrials.gov from inception until November 2022. The study protocol was published in OSF. Two independent reviewers evaluated the studies and extracted data. The quality of studies was assessed using the modified Newcastle-Ottawa scale adjusted for proteomic research. Ten studies fulfilled the protocol criteria, identifying 3328 proteins, 145 of which were differentially expressed among patients with FMS against controls. The proteins were identified in plasma, serum, cerebrospinal fluid, and saliva samples. The control groups included healthy individuals and patients with pain (inflammatory and non-inflammatory). The most important proteins identified involved transferrin, α-, β-, and γ-fibrinogen chains, profilin-1, transaldolase, PGAM1, apolipoprotein-C3, complement C4A and C1QC, immunoglobin parts, and acute phase reactants. Weak correlations were observed between proteins and pain sensation, or quality of life scales, apart from the association of transferrin and a2-macroglobulin with moderate-to-severe pain sensation. The quality of included studies was moderate-to-good. FMS appears to be related to protein dysregulation in the complement and coagulation cascades and the metabolism of iron. Several proteins may be dysregulated due to the excessive oxidative stress response.