PDF(Pubmed)
Abstract：
Background: Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder, the etiology of which has not been clearly determined yet. There is increasing evidence that synaptic and dendritic changes are involved in the etiology of ASD. The aim of this study is to determine whether serum Thrombospondin-1 and Thrombospondin-2 differ between ASD patients and healthy controls. This study also investigates possible correlations between clinical symptomatology of ASD and serum Thrombospondin-1 and Thrombospondin-2 levels. Method: A total of 44 children with ASD and 21 healthy controls under 6 years of age were included in the study. Symptom severity and behavioral problems among children with ASD were evaluated by using Childhood Autism Rating Scale and Abnormal Behavior Checklist. Serum levels of Thrombospondin-1 and Thrombospondin-2 were measured by using commercial enzyme-linked immunosorbent assay kits. Result: No statistically significant differences were found between the two groups in terms of serum Thrombospondin-1 and Thrombospondin-2 levels. In addition, no correlation was determined between Thrombospondin-2 levels and clinical symptomatology and severity of ASD. However, the Thrombospondin-1 level was found to negatively correlated with the total score of Childhood Autism Rating Scale, inappropriate speech and stereotype subscale scores of Aberrant Behavior Checklist scale. Conclusion: Thrombospondin-1 might have a potential role in the etiopathogenesis of ASD. Further studies are required to clearly elucidate the association between Trombospondin-1 and ASD.
摘要：
背景：自闭症谱系障碍（ASD）是一种神经发育障碍，其病因尚未明确确定。越来越多的证据表明，突触和树突状变化与ASD的病因有关。这项研究的目的是确定ASD患者和健康对照之间的血清血小板反应蛋白1和血小板反应蛋白2是否存在差异。这项研究还调查了ASD的临床症状学与血清血小板反应蛋白1和血小板反应蛋白2水平之间的可能相关性。方法:纳入44例ASD患儿和21例6岁以下健康对照者。采用儿童孤独症评定量表和异常行为检查表对ASD患儿的症状严重程度和行为问题进行评价。使用商业酶联免疫吸附测定试剂盒测量血小板反应蛋白-1和血小板反应蛋白-2的血清水平。结果:两组血清血小板反应蛋白-1和血小板反应蛋白-2水平差异无统计学意义。此外,血小板反应蛋白-2水平与ASD的临床症状和严重程度之间没有相关性。然而,血小板反应蛋白-1水平与儿童自闭症量表总分呈负相关，异常行为检查表量表的不适当言语和刻板印象子量表得分。结论:血小板反应素-1可能在ASD的发病机制中起潜在作用。需要进一步的研究来清楚地阐明Trombospondin-1和ASD之间的关联。
