背景:与硬化性腹膜炎(LTSP)相关的黄体化膜瘤和膜瘤之间的相似性,导致临床鉴别诊断困难。为了改善这种状况,我们选择了10个在卵巢性索-间质肿瘤临床病理学中经常使用的特定分子病理学标志物,以确定它们是否具有区分作用.
方法:应用免疫组织化学,我们分析了α-1,6-甘露糖糖蛋白6-β-n-乙酰氨基葡萄糖转移酶B(MGAT5B)的表达,核受体共激活因子3(NCOA3),增殖标记蛋白Ki-67(MKI67),雌激素受体,孕激素受体,Vimentin,受体酪氨酸蛋白激酶erbB-2,连环蛋白β-1(β-连环蛋白),CD99抗原(CD99)和Wilms肿瘤卵白(WT1)在102例疾病中包含11例LTSP和91例肿瘤。使用全外显子组测序和荧光原位杂交来检测LTSP中的MGAT5B-NCOA3融合基因。统计学分析采用t检验,方差检验的单向分析,和事后测试。
结果:验证了LTSP和thecoma之间的区别的六个重要标记,包含4个上调指标MGAT5B,NCOA3,MKI67,β-连环蛋白,和2个在黄体化细胞中下调标志物CD99和WT1。此外,MGAT5B-NCOA3融合基因首次在LTSP中被鉴定为与肿瘤相比具有显著丰富的表达。
结论:我们验证了含有MGAT5B的6个重要的分子病理标记,NCOA3,MKI67,β-连环蛋白,CD99和WT1并鉴定了LTSP中的MGAT5B-NCOA3融合基因;这项工作将帮助临床医生区分医疗状况并准确治疗患者。
BACKGROUND: Similarities between luteinized
thecoma associated with sclerosing peritonitis (LTSP) and
thecoma, cause difficulty in clinical differential diagnoses. To improve the situation, we selected 10 specified molecular pathological markers that are frequently used in clinical pathology of ovarian sex cord-stromal tumors to determine whether they exert a discriminatory effect.
METHODS: Applying immunohistochemistry, we analyzed the expression of alpha-1,6-mannosylglycoprotein 6-beta-n-acetylglucosaminyltransferase B (MGAT5B), nuclear receptor coactivator 3 (NCOA3), proliferation marker protein Ki-67 (MKI67), estrogen receptor, progesterone receptor, Vimentin, receptor tyrosine-protein kinase erbB-2, Catenin beta-1 (β-Catenin), CD99 antigen (CD99) and Wilms tumor protein (WT1) in 102 cases of diseases containing 11 LTSP and 91
thecoma. Whole-exome sequencing and fluorescence in situ hybridization were used to examine the MGAT5B-NCOA3 fusion gene in LTSP. Statistical analysis was performed using t test, one-way analysis of variance test, and post hoc test.
RESULTS: Six significant markers were verified for the discrimination between LTSP and
thecoma, containing 4 upregulating indicators MGAT5B, NCOA3, MKI67, β-Catenin, and 2 downregulating markers CD99 and WT1 in luteinized cells. In addition, the MGAT5B-NCOA3 fusion gene was identified in LTSP for the first time with significantly rich expression compared to
thecoma.
CONCLUSIONS: We verified 6 significant molecular pathological markers containing MGAT5B, NCOA3, MKI67, β-Catenin, CD99, and WT1 and identified MGAT5B-NCOA3 fusion gene in LTSP; this work will help clinicians to discriminate between medical conditions and treat patients accurately.