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Abstract：
Sclerosing stromal tumors of the ovary are benign and tend to occur in youthful women with lobular structures at low frequencies. Three types of cells, including luteinized cells, short spindle myoid cells, and intermediate cells, are found in the lobules which abundant in the blood vessels. Currently, immunohistochemistry is used to detect normal follicles, sclerosing stromal tumors, granulosa cell tumors, and fibromas/thecomas. Our research results showed that transcription factor enhancer 3 (TFE3) was moderate to strong positive in the theca interna layer of normal follicles. TFE3 was expressed in seven out of eight sclerosing stromal tumors, mainly in luteinized cells. It did not express in 20 granulosa cell tumors. Of the nine fibromas/thecomas, TFE3 was weakly staining in 2 cases and negative in the remaining 7 cases. The expression of TFE3 was also weak in only one microcystic stromal tumor. 8 cases of sclerosing stromal tumors were analyzed by FISH using a TFE3 separation probe, and the results were negative. In short, as a nuclear transcription protein, TFE3 specifically expressed in sclerosing stromal tumors and could serve as a new marker for the diagnosis and differential diagnosis of sclerosing stromal tumors. Moreover, we speculate that TFE3 will promotes the formation of the vascular plexus after entry into the nucleus, which can further explain why sclerosing stromal tumors are different from other ovary sex-cord stromal tumors.
摘要：
卵巢的硬化性间质瘤是良性的，并且倾向于在低频率下发生在具有小叶结构的年轻女性中。三种类型的细胞，包括黄体细胞,短梭形肌样细胞，和中间细胞，在血管中丰富的小叶中发现。目前,免疫组织化学用于检测正常卵泡，硬化性间质瘤,颗粒细胞瘤,和纤维瘤/囊瘤。我们的研究结果表明，转录因子增强子3（TFE3）在正常卵泡的卵泡膜层中呈中度至强阳性。TFE3在八个硬化性间质瘤中的七个中表达，主要在黄素化细胞中。它在20个颗粒细胞肿瘤中不表达。在九个纤维瘤中，2例TFE3呈弱染色，其余7例呈阴性。TFE3的表达在仅一个微囊性间质瘤中也较弱。采用TFE3分离探针对8例硬化性间质瘤进行FISH分析,结果是阴性。总之,作为核转录蛋白，TFE3在硬化性间质瘤中特异性表达，可作为诊断和鉴别诊断硬化性间质瘤的新标志物。此外，我们推测TFE3进入细胞核后会促进血管丛的形成，这可以进一步解释为什么硬化性间质瘤不同于其他卵巢性索间质瘤。
