茶氨酸,多酚,咖啡因不仅影响茶的味道,而且在人类健康方面也起着重要的作用。然而,SeNMs对降脂成分的具体调控机制尚不清楚。在这项研究中,研究了福鼎大白(FDDB)茶中降脂成分的合成。结果表明,100芽的重量,茶氨酸,EGCG,总儿茶素,10mg·L-1SeNMs在24.3%的范围内最佳地促进了茶芽中的咖啡因含量,36.2%,53.9%,67.1%,和30.9%,分别。机械上,SeNMs促进茶树光合作用,增加了茶叶中的可溶性糖含量(30.3%),并为代谢过程提供能量,包括TCA循环,丙酮酸代谢,氨基酸代谢,和谷氨酰胺/谷氨酸循环,最终增加茶芽中氨基酸和抗氧化物质(儿茶素)的含量;儿茶素合成关键基因的相对表达,CsPAL,CsC4H,CsCHI,CsDFR,CsANS,CsANR,CsLAR,和UGGT,显着上调了45.1-619.1%。茶氨酸合成基因CsTs的表达,CsG,CsGOGAT上调138.8-693.7%。此外,SeNMs促进了更多的蔗糖转移到根部,CsSUT1、CsSUT2、CsSUT3和CsSWEET1a上调125.8-560.5%。相应地,SeNMs丰富了有益的根际微生物群(Roseiarcus,酸热,嗜酸杆菌,词典,和Pedosphaeraceae),提高茶树对铵态氮的吸收和利用,有助于茶氨酸的积累。这项研究提供了令人信服的证据,支持SeNMs通过增强氮代谢来促进茶的降脂成分。
Theanine, polyphenols, and caffeine not only affect the flavor of tea, but also play an important role in human health benefits. However, the specific regulatory mechanism of Se NMs on fat-reducing components is still unclear. In this study, the synthesis of fat-reducing components in Fuding Dabai (FDDB) tea was investigated. The results indicated that the 100-bud weight,
theanine, EGCG, total catechin, and caffeine contents of tea buds were optimally promoted by 10 mg·L-1 Se NMs in the range of 24.3%, 36.2%, 53.9%, 67.1%, and 30.9%, respectively. Mechanically, Se NMs promoted photosynthesis in tea plants, increased the soluble sugar content in tea leaves (30.3%), and provided energy for the metabolic processes, including the TCA cycle, pyruvate metabolism, amino acid metabolism, and the glutamine/glutamic acid cycle, ultimately increasing the content of amino acids and antioxidant substances (catechins) in tea buds; the relative expressions of key genes for catechin synthesis, CsPAL, CsC4H, CsCHI, CsDFR, CsANS, CsANR, CsLAR, and UGGT, were significantly upregulated by 45.1-619.1%. The expressions of
theanine synthesis genes CsTs, CsGs, and CsGOGAT were upregulated by 138.8-693.7%. Moreover, Se NMs promoted more sucrose transfer to the roots, with the upregulations of CsSUT1, CsSUT2, CsSUT3, and CsSWEET1a by 125.8-560.5%. Correspondingly, Se NMs enriched the beneficial rhizosphere microbiota (Roseiarcus, Acidothermus, Acidibacter, Conexicter, and Pedosphaeraceae), enhancing the absorption and utilization of ammonium nitrogen by tea plants, contributing to the accumulation of
theanine. This study provides compelling evidence supporting the application of Se NMs in promoting the lipid-reducing components of tea by enhancing its nitrogen metabolism.