Abstract：
Acrolein (ACR), methylglyoxal (MGO), and glyoxal (GO) are a class of reactive carbonyl species (RCS), which play a crucial role in the pathogenesis of chronic and age-related diseases. Here, we explored a new RCS inhibitor (theanine, THE) and investigated its capture capacity on RCS in vivo by human experiments. After proving that theanine could efficiently capture ACR instead of MGO/GO by forming adducts under simulated physiological conditions, we further detected the ACR/MGO/GO adducts of theanine in the human urine samples after consumption of theanine capsules (200 and 400 mg) or green tea (4 cups, containing 200 mg of theanine) by using ultraperformance liquid chromatography-time-of-flight-high-resolution mass spectrometry. Quantitative assays revealed that THE-ACR, THE-2ACR-1, THE-MGO, and THE-GO were formed in a dose-dependent manner in the theanine capsule groups; the maximum value of the adducts of theanine was also tested. Furthermore, besides the RCS adducts of theanine, the RCS adducts of catechins could also be detected in the drinking tea group. Whereas, metabolite profile analysis showed that theanine could better capture RCS produced in the renal metabolic pathway than catechins. Our findings indicated that theanine could reduce RCS in the body in two ways: as a pure component or contained in tea leaves.
摘要：
丙烯醛(ACR),甲基乙二醛（MGO），乙二醛（GO）是一类反应性羰基（RCS），在慢性和年龄相关疾病的发病机制中起着至关重要的作用。这里,我们探索了一种新的RCS抑制剂(茶氨酸，THE)，并通过人体实验研究了其在体内对RCS的捕获能力。证明茶氨酸在模拟生理条件下通过形成加合物可以有效捕获ACR而不是MGO/GO,我们在食用茶氨酸胶囊（200和400毫克）或绿茶（4杯，含有200毫克茶氨酸），使用超高效液相色谱-飞行时间-高分辨率质谱。定量分析显示，THE-ACR，THE-2ACR-1,THE-MGO,和THE-GO在茶氨酸胶囊组中以剂量依赖性方式形成;还测试了茶氨酸加合物的最大值。此外，除了茶氨酸的RCS加合物，在饮茶组中也可以检测到儿茶素的RCS加合物。然而,代谢物谱分析表明茶氨酸比儿茶素能更好地捕获肾代谢途径中产生的RCS。我们的发现表明，茶氨酸可以通过两种方式减少体内的RCS：作为纯成分或包含在茶叶中。
