背景:牛肌肉的大理石花纹特征,作为一个关键指标,在牛肉品质评价中发挥了重要作用。两种牛,即高大理石花纹(安格斯)和低大理石花纹(南阳),他们的牛肌肉被选为我们的转录组测序样本,旨在鉴定差异表达的长链非编码RNA(lncRNA)及其与大理石花纹性状相关的靶标。
结果:转录组测序在高大理石纹(安格斯)和低大理石纹(南阳)牛肌肉之间分别鉴定了487和283个差异表达的mRNA和lncRNA。二十七对差异表达的lncRNAs-mRNAs,包括18个lncRNAs和11个靶基因,被发现参与脂肪沉积和脂质代谢。我们建立了14个上调的基因(NONBTAT000849.2,MSTRG.951.1,NONBTAT031089.1,MSTRG.3720.1,NONBTAT029718.1,NONBTAT004228.2,NONBTAT007494.2,NOBTAT011094.2,NOBTAT1815080.2,NOBTNOBTNpO182T80.2。细胞色素P450家族(CYP4V2),3-羟酰基辅酶A脱水酶家族(HACD4),kinesin家族(KIF5C),lipin家族(LPIN2),perilipin系列(PLIN1),前列腺素家族(PTGIS),溶质载体家族(SLC16A7、SLC2213和SLCO4C1),并含有跨膜结构域蛋白家族(VSTM1)。
结论:这些候选基因和lncRNAs可被视为负责调节牛的大理石花纹性状。lncRNAs以及肌内脂肪大理石花纹的变异为阐明牛高大理石花纹的遗传基础奠定了基础。
BACKGROUND: The marbling trait of cattle muscles, being a key indicator, played an important role in evaluating beef quality. Two breeds of cattle, namely a high-marbling (Angus) and a low-marbling (Nanyang) one, with their cattle muscles selected as our samples for transcriptome sequencing, were aimed to identify differentially expressed long non-coding RNAs (lncRNAs) and their targets associated with the marbling trait.
RESULTS: Transcriptome sequencing identified 487 and 283 differentially expressed mRNAs and lncRNAs respectively between the high-marbling (Angus) and low-marbling (Nanyang) cattle muscles. Twenty-seven pairs of differentially expressed lncRNAs-mRNAs, including eighteen lncRNAs and eleven target genes, were found to be involved in fat deposition and lipid metabolism. We established a positive correlation between fourteen up-regulated (NONBTAT000849.2, MSTRG.9591.1, NONBTAT031089.1, MSTRG.3720.1, NONBTAT029718.1, NONBTAT004228.2, NONBTAT007494.2, NONBTAT011094.2, NONBTAT015080.2, NONBTAT030943.1, NONBTAT021005.2, NONBTAT021004.2, NONBTAT025985.2, and NONBTAT023845.2) and four down-regulated (NONBTAT000850.2, MSTRG.22188.3, MSTRG.22188.4, and MSTRG.22188.5) lncRNAs and eleven genes related to adiponectin family protein (ADIPOQ), cytochrome P450 family (CYP4V2), 3-hydroxyacyl-CoA dehydratase family (HACD4), kinesin family (KIF5C), lipin family (LPIN2), perilipin family (PLIN1), prostaglandin family (PTGIS), solute carrier family (SLC16A7, SLC2213, and SLCO4C1), and containing a transmembrane domain protein family (VSTM1).
CONCLUSIONS: These candidate genes and lncRNAs can be regarded as being responsible for regulating the marbling trait of cattle. lncRNAs along with the variations in intramuscular fat marbling established a foundation for elucidating the genetic basis of high marbling in cattle.