Abstract：
Drought stress strongly affects crop yield. Although knowledge of long non-coding RNAs (lncRNAs) has been updated continuously and rapidly, information about lncRNAs in drought resistance regulation is extremely limited in sorghum. Here, lncRNA-sequencing was performed with seedlings of a sorghum cultivar (Jinza29) under three water control treatments to investigate the mechanism of lncRNAs responsible for drought resistance in sorghum. A total of 377 differentially expressed lncRNAs (DElncRNAs) were identified. We also predicted 4322 and 2827 transcripts as potential cis-target and trans-target genes for drought-responsive lncRNAs, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that those target genes exhibited marked enrichment into \"oxidoreductase activity\", \"signal transducer activity\", \"DNA repair\", \"photosynthesis\", \"glutathione metabolism\", and \"phenylpropanoid biosynthesis\" and other terms associated with abiotic stress resistance. Moreover, several lncRNAs were estimated to modulate the expression of other genes related to stress response and photosynthetic carbon metabolism. Additionally, we found 107 DElncRNAs that might be candidate target mimics for 56 miRNAs. LncRNAs play important roles in drought adaptation of sorghum through interacting with protein-encoding genes. The obtained results provided novel insights into the biological characteristics of lncRNAs and offered potential regulatory factors for genetically enhancing drought resistance in sorghum.
摘要：
干旱胁迫强烈影响作物产量。尽管长非编码RNA（lncRNAs）的知识已经不断更新，关于lncRNAs在高粱抗旱性调控中的信息非常有限。这里,在三种水控制处理下，对高粱品种（Jinza29）的幼苗进行lncRNA测序，以研究lncRNA对高粱抗旱性的机制。鉴定了总共377种差异表达的lncRNA(DElncRNA)。我们还预测了4322和2827个转录本作为干旱响应性lncRNAs的潜在顺式靶和反式靶基因。分别。基因本体论（GO）和京都基因和基因组百科全书（KEGG）分析表明，这些靶基因表现出明显的“氧化还原酶活性”富集，\"信号传感器活动\",\"DNA修复\",\"光合作用\",“谷胱甘肽代谢”，以及“苯丙素生物合成”和其他与非生物胁迫抗性相关的术语。此外，估计有几个lncRNAs可以调节与胁迫反应和光合碳代谢相关的其他基因的表达。此外,我们发现107个DElncRNAs可能是56个miRNAs的候选靶模拟物。LncRNAs通过与蛋白质编码基因的相互作用在高粱的干旱适应中发挥重要作用。获得的结果为lncRNAs的生物学特性提供了新的见解，并为高粱的遗传增强抗旱性提供了潜在的调节因子。
