背景:鸟分枝杆菌亚种副结核(MAP)在亚临床阶段的奶牛中引起慢性和进行性肉芽肿性肠炎和经济损失。可以使用血清MAP抗体酶联免疫吸附测定(ELISA)和粪便聚合酶链反应(PCR)测试来检测牛的亚临床感染。
目的:为了研究血液参数的差异,根据血清抗体ELISA和粪便PCR检测MAP。
方法:我们将33头亚临床感染的成年牛分为三组:血清阴性和粪便阳性(SNFP,n=5),血清阳性和粪便阴性(SPFN,n=10),血清阳性和粪便阳性(SPFP,n=18)。进行血液学和血清生化分析。
结果:虽然奶牛在临床上是健康的,没有任何表现,SNFP和SPFP组有更高的血小板计数,平均血小板体积,Plateletcrit,乳酸脱氢酶水平,乳酸水平,和钙水平,但平均红细胞体积浓度低于SPFN组(p<0.017)。红细胞计数,血细胞比容,单核细胞计数,葡萄糖水平,根据检测方法不同,钙卫蛋白水平不同(p<0.05)。SNFP和SPFP组有较高的红细胞计数,血细胞比容和钙卫蛋白水平,但单核细胞计数和葡萄糖水平低于SPFN组,尽管没有显着差异(p>0.017)。
结论:粪便MAP阳性的母牛与粪便MAP阴性的母牛的血液参数不同,尽管他们是亚临床感染。这些发现为了解亚临床感染牛的MAP感染机制提供了新的见解。
BACKGROUND: Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic and progressive granulomatous enteritis and economic losses in dairy cattle in subclinical stages. Subclinical infection in cattle can be detected using serum MAP antibody enzyme-linked immunosorbent assay (ELISA) and fecal polymerase chain reaction (PCR) tests.
OBJECTIVE: To investigate the differences in blood parameters, according to the detection of MAP using serum antibody ELISA and fecal PCR tests.
METHODS: We divided 33 subclinically infected adult cattle into three groups: seronegative and fecal-positive (SNFP, n = 5), seropositive and fecal-negative (SPFN, n = 10), and seropositive and fecal-positive (SPFP, n = 18). Hematological and serum biochemical analyses were performed.
RESULTS: Although the cows were clinically healthy without any manifestations, the SNFP and SPFP groups had higher platelet counts, mean platelet volumes, plateletcrit, lactate dehydrogenase levels, lactate levels, and calcium levels but lower mean corpuscular volume concentration than the SPFN group (p < 0.017). The red blood cell count, hematocrit, monocyte count, glucose level, and calprotectin level were different according to the detection method (p < 0.05). The SNFP and SPFP groups had higher red blood cell counts, hematocrit and calprotectin levels, but lower monocyte counts and glucose levels than the SPFN group, although there were no significant differences (p > 0.017).
CONCLUSIONS: The cows with fecal-positive MAP status had different blood parameters from those with fecal-negative MAP status, although they were subclinically infected. These findings provide new insights into understanding the mechanism of MAP infection in subclinically infected cattle.