PDF(Pubmed)
Abstract：
Lumpy skin disease virus (LSDV) is a vector-transmitted capripox virus that causes disease in cattle. Stomoxys calcitrans flies are considered to be important vectors as they are able to transmit viruses from cattle with the typical LSDV skin nodules to naive cattle. No conclusive data are, however, available concerning the role of subclinically or preclinically infected cattle in virus transmission. Therefore, an in vivo transmission study with 13 donors, experimentally inoculated with LSDV, and 13 naïve acceptor bulls was performed whereby S. calcitrans flies were fed on either subclinical- or preclinical-infected donor animals. Transmission of LSDV from subclinical donors showing proof of productive virus replication but without formation of skin nodules was demonstrated in two out of five acceptor animals, while no transmission was seen from preclinical donors that developed nodules after Stomoxys calcitrans flies had fed. Interestingly, one of the acceptor animals which became infected developed a subclinical form of the disease. Our results show that subclinical animals can contribute to virus transmission. Therefore, stamping out only clinically diseased LSDV-infected cattle could be insufficient to completely halt the spread and control of the disease.
摘要：
结节性皮肤病病毒（LSDV）是一种媒介传播的羊痘病毒，可在牛中引起疾病。Stomoxys骨化果蝇被认为是重要的载体，因为它们能够将病毒从具有典型LSDV皮肤结节的牛传播给幼稚的牛。没有确凿的数据，然而,关于亚临床或临床前感染的牛在病毒传播中的作用。因此,对13个供体进行的体内传播研究，实验接种LSDV，并进行了13只天然受体公牛，其中将钙绿链球菌蝇饲喂至亚临床或临床前感染的供体动物。来自亚临床供体的LSDV的传播，显示出生产性病毒复制的证据，但没有形成皮肤结节，在五个受体动物中的两个中得到证实。而在StomoxysCalcitrans果蝇喂食后出现结节的临床前供体没有发现传播。有趣的是,被感染的受体动物之一发展成亚临床形式的疾病。我们的结果表明，亚临床动物可以促进病毒传播。因此,仅剔除临床上感染LSDV的牛可能不足以完全阻止疾病的传播和控制。
