目的:在全球范围内连续报道了与禽肉有关的沙门氏菌暴发。因此,屠宰场禽肉的沙门氏菌污染是减少人类疾病爆发的关键控制点之一。
目的:这项研究检查了韩国整个屠宰过程中沙门氏菌物种的残留污染情况。
方法:从2018年到2019年,从全国分布的9个屠宰场收集了1,097个样本。使用invA基因特异性聚合酶链反应鉴定了一百一十七株沙门氏菌。如前所述。血清型,系统发育,并检查了分离株的耐药性。
结果:在117个分离株中,93例血清分型为沙门氏菌Mbandaka(n=36株,30.8%),汤普森沙门氏菌(n=33,28.2%),和沙门氏菌婴儿(n=24,20.5%)。有趣的是,等位基因分析表明,所有S.Mbandaka分离株都属于序列类型(ST)413的谱系,而所有S.Thompson分离株都是ST292。此外,几乎所有S.Thompson分离株(97.0%,属于ST292的32/33分离株)具有多重耐药性,并具有主要的毒力基因,其产物是完全毒力所必需的。两种血清型在整个屠宰过程中广泛分布。脉冲场凝胶电泳分析表明,七个婴儿在其系统发育亲缘关系中显示出100%的同一性,表明它们是在屠宰过程中依次传播的。
结论:这项研究提供了更多的证据,证明了沙门氏菌在屠宰过程中的残留传播。ST292S.Thompson是沙门氏菌的潜在致病性克隆,可能与韩国的食源性暴发有关。
OBJECTIVE: Salmonella outbreaks linked to poultry meat have been reported continuously worldwide. Therefore, Salmonella contamination of poultry meats in slaughterhouses is one of the critical control points for reducing disease outbreaks in humans.
OBJECTIVE: This study examined the carry-over contamination of Salmonella species through the entire slaughtering process in South Korea.
METHODS: From 2018 to 2019, 1,097 samples were collected from the nine slaughterhouses distributed nationwide. One hundred and seventeen isolates of Salmonella species were identified using the invA gene-specific polymerase chain reaction, as described previously. The serotype, phylogeny, and antimicrobial resistance of isolates were examined.
RESULTS: Among the 117 isolates, 93 were serotyped into Salmonella Mbandaka (n = 36 isolates, 30.8%), Salmonella Thompson (n = 33, 28.2%), and Salmonella Infantis (n = 24, 20.5%). Interestingly, allelic profiling showed that all S. Mbandaka isolates belonged to the lineage of the sequence type (ST) 413, whereas all S. Thompson isolates were ST292. Moreover, almost all S. Thompson isolates (97.0%, 32/33 isolates) belonging to ST292 were multidrug-resistant and possessed the major virulence genes whose products are required for full virulence. Both serotypes were distributed widely throughout the slaughtering process. Pulsed-field gel electrophoretic analysis demonstrated that seven S. Infantis showed 100% identities in their phylogenetic relatedness, indicating that they were sequentially transmitted along the slaughtering processes.
CONCLUSIONS: This study provides more evidence of the carry-over transmission of Salmonella species during the slaughtering processes. ST292 S. Thompson is a potential pathogenic clone of Salmonella species possibly associated with foodborne outbreaks in South Korea.