PDF(Pubmed)
Abstract：
Salmonella diarizonae (IIIb) is frequently isolated from reptiles and less frequently from birds and mammals. However, its isolation from invasive human infections has not been widely reported. Migratory mallard ducks are excellent bioindicators of pathogen presence and pathogen antibiotic resistance (AMR). We present the first isolation from a mallard duck in central Europe of the antibiotic-resistant Salmonella enterica subsp. diarizonae with the unique antigenic pattern 58:r:z53 and report its whole-genome sequencing, serosequencing, and genotyping, which enabled the prediction of its pathogenicity and comparison with phenotypic AMR. The isolated strain was highly similar to S. diarizonae isolated from humans and food. Twenty-four AMR genes were detected, including those encoding aminoglycoside, fluoroquinolone, macrolide, carbapenem, tetracycline, cephalosporin, nitroimidazole, peptide antibiotic, and disinfecting agent/antiseptic resistance. Six Salmonella pathogenicity islands were found (SPI-1, SPI-2, SPI-3, SPI-5, SPI-9, and SPI-13). An iron transport system was detected in SPI-1 centisome C63PI. Plasmid profile analyses showed three to be present. Sequence mutations in the invA and invF genes were noted, which truncated and elongated the proteins, respectively. The strain also harbored genes encoding type-III secretion-system effector proteins and many virulence factors found in S. diarizonae associated with human infections. This study aims to elucidate the AMR and virulence genes in S. enterica subsp. diarizonae that may most seriously threaten human health.
摘要：
沙门氏菌（IIIb）通常从爬行动物中分离出来，而从鸟类和哺乳动物中分离出来的频率较低。然而,它与人类侵袭性感染的分离尚未得到广泛报道。迁徙野鸭是病原体存在和病原体抗生素抗性（AMR）的出色生物指标。我们介绍了从中欧一只野鸭中首次分离出的耐抗生素沙门氏菌肠亚种。具有独特抗原模式58：r：z53的dirizonae，并报告了其全基因组测序，血清测序，和基因分型，这使得能够预测其致病性并与表型AMR进行比较。分离出的菌株与从人类和食物中分离出的S.diarizonae高度相似。检测到24个AMR基因，包括那些编码氨基糖苷的,氟喹诺酮,大环内酯,碳青霉烯,四环素,头孢菌素,硝基咪唑，肽抗生素，和消毒剂/防腐剂抗性。发现了六个沙门氏菌致病性岛（SPI-1，SPI-2，SPI-3，SPI-5，SPI-9和SPI-13）。在SPI-1厘米C63PI中检测到铁转运系统。质粒概况分析显示存在三个。注意到invA和invF基因的序列突变，截短和延长蛋白质，分别。该菌株还包含编码III型分泌系统效应蛋白的基因和在与人类感染相关的Diarizonae中发现的许多毒力因子。本研究旨在阐明肠球菌亚种中的AMR和毒力基因。可能对人类健康造成最严重的威胁。
