Abstract：
Detection of Salmonella sp. is important for the broiler chicken production chain because it is one microorganisms involved in food-borne diseases. Thus, this study performed the optimization of a technique of Loop-mediated isothermal DNA amplification (LAMP) through the 3MTM Molecular Detection Assay 2: Salmonella (MDS®), in accordance with Ordinance number 126 of the Ministry of Agriculture, for the detection of Salmonella sp. in drag swab. The methodology followed ISO 16140-2: 2016, with the analysis naturally contaminated drag swab samples collected from broiler aviaries and artificially contaminated with salmonella ATCCs. Of the 300 samples processed in protocol A (pre-enrichment tetrathionate broth (TT)), 45 were positive for Salmonella sp., 242 negative, one false-positive, and 12 false-negative, while of the 300 samples analyzed in protocol B (pre-enrichment brain-heart infusion broth (BHI)), 40 were positive, 256 negative, one false-positive, and three false-negative. The result for protocol A was a sensitivity of 79%, specificity of 99.6%, Positive Predictive Value (PPV) of 98%, and Negative Predictive Value (NPV) of 95%; and for protocol B, 93% sensitivity, 99.6% specificity, 98% PPV, and 99% NPV. Both protocols were associated with the reference method (p>0.05), concluding that the MDS® can be used for the qualitative detection of Salmonella sp.
摘要：
沙门氏菌的检测。对肉鸡生产链很重要，因为它是一种涉及食源性疾病的微生物。因此，本研究通过3MTM分子检测分析2：沙门氏菌（MDS®）对环介导等温DNA扩增（LAMP）技术进行了优化，根据农业部第126号条例，用于检测沙门氏菌。在拖拽棉签中。该方法遵循ISO16140-2：2016，分析从肉鸡鸟舍收集的自然污染的拭子样品，并人工污染沙门氏菌ATCC。在方案A（预富集四硫酸盐肉汤（TT））中处理的300个样品中，45对沙门氏菌呈阳性。,242负,一个假阳性,和12个假阴性，而在方案B中分析的300个样品（富集前脑心输注肉汤（BHI）），40是积极的，256个阴性,一个假阳性,和三个假阴性。方案A的结果是79%的灵敏度,特异性99.6%，正预测值（PPV）为98%，和95%的负预测值(NPV)；对于方案B，93%灵敏度,99.6%的特异性，98%PPV,和99%的净现值。两种方案均与参考方法相关(p>0.05)，结论是MDS®可用于沙门氏菌的定性检测。
