背景:作为生物体中必不可少的化合物,糖类引起了各个领域科学家的极大关注。了解糖在各种样品中的分布具有重要的科学意义。然而,糖类的低信号响应和特异性识别技术的缺乏以及样品的复杂基质使得糖类的分析成为一项非常具有挑战性的任务。因此,开发一种简单而直接的糖分析策略将对该领域做出巨大贡献。
结果:在这项研究中,通过使用磺酰基官能化的磁性树枝状介孔二氧化硅纳米颗粒作为基底,我们开发了一个分析糖类的综合平台。平台的建设主要依靠多功能硼酸,它同时充当分离和衍生配体。在一般程序中,首先将硼酸固定在基材表面,然后通过硼酸酯亲和分离实现糖类的选择性富集。最后,通过对解决方案的理性选择,我们能够从底物中洗脱标记的复合物(硼酸-糖),可以直接进行HPLC-UV分析。可靠的精度(<15%),准确度(80-100%),重现性(<10%),实验证明了所提出平台的改进的灵敏度(20x)和有限的耗时(低至几分钟)。
■不同糖类(糖醇,葡萄糖)在真实样品中实现。拟议的战略不仅简单明了,而且快速,还要避免对专用设备的要求。有了这些吸引人的特点,我们相信这种策略将极大地促进各种样品中糖类的分析(例如。食物,药物和生物样品)。
BACKGROUND: As an essential compound in living organism,
saccharides have attracted enormous attentions from scientists in various fields. Understanding the distribution of
saccharides in various samples is of great scientific importance. However, the low signal response and lack of specific recognition technology of
saccharides and the complex matrix of samples make the analysis of
saccharides a very challenge task. Thus, the development of a simple and straightforward strategy for the analysis of
saccharides would represent a great contribution to the field.
RESULTS: In this study, by employing the sulfonyl functionalized magnetic dendritic mesoporous silica nanoparticles as the substrate, we develop an integrated platform for analysis of saccharides. The construction of the platform mainly relied on multi-functional boronic acid, which serves as separation and derivation ligands at the same time. In the general procedure, the boronic acid is first immobilized onto the surface of substrate, then the selective enrichment of saccharides can be realized via boronate affinity separation. Finally, by the rational choice of the solution, we are able to elute the labelled complex (boronic acid-saccharide) from the substrate, which can be direct subjected to HPLC-UV analysis. The reliable precision (<15 %), accuracy (80-100 %), reproducibility (<10 %), improved sensitivity (20x) and limited time-consuming (down to minutes) of the proposed platform are experimentally demonstrated.
UNASSIGNED: The successful quantification of different saccharides (alditols, glucose) in real samples is achieved. The proposed strategy is not only straightforward and fast, but also avoid the requirement of special equipment. With these attractive features, we believe that this strategy will greatly prompt the analysis of saccharides in various samples (eg. food, pharmaceutics and biosamples).