Abstract：
Aim: Bioanalytical assays to measure rhamnose, erythritol, lactulose and sucralose in human urine and plasma were developed to support an indomethacin challenge study for intestinal permeability assessment in healthy participants. Methods: The multi-sugar assays utilized 5-μl sample matrix and a simple chemical derivatization with acetic anhydride, followed by RPLC-MS/MS detection. Results: Rhamnose and erythritol quantification was established between 1.00-1,000 μg/ml in urine and 250-250,000 ng/ml in plasma. For lactulose and sucralose, dynamic ranges of 0.1-100 μg/ml (urine) and 25-25,000 ng/ml (plasma) were applied for biological measurements. Conclusion: This work helped overcome some of the common analytical challenges associated with the bioanalysis of mono- and disaccharides and achieved improved limits of quantification.
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[Box: see text].
摘要：
目的：生物分析测定鼠李糖，赤藓糖醇,人体尿液和血浆中的乳果糖和三氯蔗糖被开发用于支持吲哚美辛挑战研究，以评估健康参与者的肠道通透性。方法：多糖测定法利用5μl样品基质和简单的乙酸酐化学衍生，然后进行RPLC-MS/MS检测。结果：在尿液中1.00-1,000μg/ml和血浆中250-250,000ng/ml之间建立了鼠李糖和赤藓糖醇的定量。对于乳果糖和三氯蔗糖，应用0.1-100μg/ml(尿液)和25-25,000ng/ml(血浆)的动态范围进行生物测量.结论：这项工作有助于克服与单糖和双糖生物分析相关的一些常见分析挑战，并实现了改进的定量限。
[方框：见正文]。
[方框：见正文]。
