Abstract：
Structural complexity brings a huge challenge to the analysis of sugar chains. As a single-molecule sensor, nanopores have the potential to provide fingerprint information on saccharides. Traditionally, direct single-molecule saccharide detection with nanopores is hampered by their small size and weak affinity. Here, a carbon nitride nanopore device is developed to discern two types of trisaccharide molecules (LeApN and SLeCpN) with minor structural differences. The resolution of LeApN and SLeCpN in the mixture reaches 0.98, which has never been achieved in solid-state nanopores so far. Monosaccharide (GlcNAcpN) and disaccharide (LacNAcpN) can also be discriminated using this system, indicating that the versatile carbon nitride nanopores possess a monosaccharide-level resolution. This study demonstrates that the carbon nitride nanopores have the potential for conducting structure analysis on single-molecule saccharides.
摘要：
结构复杂性给糖链的分析带来了巨大的挑战。作为单分子传感器，纳米孔具有提供糖类指纹信息的潜力。传统上,用纳米孔直接检测单分子糖受到它们的小尺寸和弱亲和力的阻碍。这里,开发了碳氮化物纳米孔装置以辨别具有微小结构差异的两种类型的三糖分子(LeApN和SLeCpN)。LeApN和SLeCpN在混合物中的分辨率达到0.98，这是迄今为止在固态纳米孔中从未实现的。单糖（GlcNAcpN）和二糖（LacNAcpN）也可以使用该系统进行区分，表明通用的碳氮化物纳米孔具有单糖水平的分辨率。这项研究表明，氮化碳纳米孔具有对单分子糖类进行结构分析的潜力。
