%0 Journal Article
%T Development and application of a multi-sugar assay to assess intestinal permeability.
%A Schneck NA
%A Moghieb A
%A Teague C
%A Perez HL
%J Bioanalysis
%V 0
%N 0
%D 2024 Jul 18
%M 39023344
%F 2.695
%R 10.1080/17576180.2024.2374168
%X Aim: Bioanalytical assays to measure rhamnose, erythritol, lactulose and sucralose in human urine and plasma were developed to support an indomethacin challenge study for intestinal permeability assessment in healthy participants. Methods: The multi-sugar assays utilized 5-μl sample matrix and a simple chemical derivatization with acetic anhydride, followed by RPLC-MS/MS detection. Results: Rhamnose and erythritol quantification was established between 1.00-1,000 μg/ml in urine and 250-250,000 ng/ml in plasma. For lactulose and sucralose, dynamic ranges of 0.1-100 μg/ml (urine) and 25-25,000 ng/ml (plasma) were applied for biological measurements. Conclusion: This work helped overcome some of the common analytical challenges associated with the bioanalysis of mono- and disaccharides and achieved improved limits of quantification.<BR>[Box: see text].