六价铬,有毒重金属,在最受关注的环境污染物中,被宣布为人类和动物的有效内分泌干扰物。本研究旨在发现Cr(VI)对生殖系统的有害影响以及Nigellasativa和Nigella介导的AgNP对雄性小鼠(Musmusculus)的改善作用。在本研究中,已知的不孕症药物,克罗米芬柠檬酸盐也用作阳性对照。本研究的主要目的是评估口服剂量为50mg/kgBW的克罗米芬(对照)的改善潜力,通过化学合成AgNP,黑麦草种子提取物,和Nigellasativa介导的AgNP以1.5mg/kgBW的剂量从K2Cr2O7口服诱导的8周内对雄性白化病小鼠的繁殖性能产生的毒性。黑麦草介导的AgNPs通过UV表征,SEM,FTIR,和XRD。组织学分析,涂片研究,抗氧化能力测试,和激素分析是通过白化病小鼠的血液样本进行的。Cr暴露组精子头部宽度(5.29±0.54µ)和长度(19.54±1.18µ)显着降低,中间片长度,尾部长度,LH(1.65±0.15ng/mL),睾酮(2.63±0.29ng/mL),SOD(61.40±2.48mmol/mL),CAT(87.40±6.01mmol/mL),GSH(1.54±0.09µmol/mL),精原细胞无(1.22±0.25),精母细胞(2.33±0.943)。然而,FSH水平(160.00±4.98ng/mL),生精管CSA(1094.69±49.76mm2),精原细胞大小(41.30±1.24µ),精母细胞(26.07±1.34µ)显着增加。施用Nigellasativa和Nigellasativa介导的AgNP降低了毒性。
Hexavalent chromium, toxic heavy metal, among the top-rated environmental contaminants, is declared a potent endocrine disruptor in humans and animals. The present study was planned to find harmful effects on the reproductive system caused by Cr (VI) and the ameliorative effect of Nigella sativa and Nigella sativa-mediated AgNP on male mice (Mus musculus). In the present study, known infertility medicine, clomiphene citrate is also used as a positive control. The main objective of the present study was to assess the ameliorative potential of oral administration of a dose of 50 mg/kg BW clomiphene citrate (control), AgNP via chemical synthesis, Nigella sativa seed extract, and Nigella sativa-mediated AgNP against the Cr (VI) at the dose of 1.5 mg/kg BW from K2Cr2O7 orally induced toxicity over eight weeks on the reproductive performance of male albino mice. Nigella sativa mediated AgNPs were characterized by UV, SEM, FTIR, and XRD. The histological analysis, smear study, antioxidant capacity test, and hormone analysis were conducted by blood samples of albino mice. Cr exposed groups showed a significant decrease in sperm head breadth (5.29 ± 0.54 µ) and length (19.54 ± 1.18 µ), middle piece length, tail length, LH (1.65 ± 0.15 ng/mL), testosterone (2.63 ± 0.29 ng/mL), SOD (61.40 ± 2.48 mmol/mL), CAT (87.40 ± 6.01 mmol/mL), GSH (1.54 ± 0.09 µmol/mL), and no of spermatogonia (1.22 ± 0.25), and spermatocytes (2.33 ± 0.943). However, FSH level (160.00 ± 4.98 ng/mL), seminiferous tubule CSA (1094.69 ± 49.76 mm2), size of spermatogonia (41.30 ± 1.24 µ), and spermatocytes (26.07 ± 1.34 µ) were significantly increased. Administration of Nigella sativa and Nigella sativa-mediated AgNPs reduced the toxicity.