PDF(Pubmed)
Abstract：
Reports about the impact of Carbon tetrachloride (CCl4) hepatotoxicity on coagulation profile have been inconsistent. Multiple investigators have however demonstrated the effectiveness of silymarin in the resolution of anomalies induced by CCl4, although the effect of silymarin on the impact of CCl4 hepatotoxicity, especially coagulation profile and osmotic fragility have not been investigated. The liver, the primary site for the secretion of coagulation proteins, can become impaired in CCl4 hepatotoxicity, and silymarin reportedly increases hepatic protein synthesis as part of its hepatoprotective mechanism. This study assessed the effect of silymarin on blood coagulation profile and erythrocyte osmotic fragility in CCl4 induced hepatotoxicity in rats. Twenty male Wistar rats were allocated into four groups (n = 5) at random, namely: Control, CCl4 given CCl4 (1 ml/kg) administered intraperitoneally twice a week, Silymarin (S) given silymarin (100 mg/kg/day) orally, and S+CCl4 given silymarin (100 mg/kg/day) orally and (1 ml/kg) CCl4 one hour after, intraperitoneally twice a week for a duration of four weeks. Results showed protraction of activated partial thromboplastin time and thrombin time, increased erythrocyte osmotic fragility, liver damage, dyslipidemia, oxidative stress and lipid peroxidation in rats given CCl4. Silymarin attenuated most of these effects as observed from comparison between CCl4 and S+CCl4 rats. The findings of this study suggests that pretreatment with silymarin attenuated disruption in coagulation profile and erythrocyte osmotic fragility in CCl4 induced hepatotoxicity in Wistar rats.
摘要：
关于四氯化碳（CCl4）肝毒性对凝血谱的影响的报道一直不一致。然而，多个研究人员已经证明了水飞蓟素在CCl4引起的异常的分辨率的有效性，尽管水飞蓟素对CCl4肝毒性的影响，特别是凝血曲线和渗透脆性尚未研究。肝脏，凝血蛋白分泌的主要部位，CCl4肝毒性可能受损，据报道，水飞蓟素增加肝蛋白合成作为其肝脏保护机制的一部分。本研究评估了水飞蓟素对CCl4诱导的大鼠肝毒性的凝血特性和红细胞渗透脆性的影响。20只雄性Wistar大鼠随机分为4组(n=5),即：控制，CCl4给予CCl4（1ml/kg），每周两次腹膜内给药，水飞蓟素（S）口服水飞蓟素（100mg/kg/天），和S+CCl4给予水飞蓟素（100毫克/千克/天）口服和（1毫升/千克）CCl4后一小时，每周两次腹膜内注射，持续四周。结果显示活化部分凝血活酶时间和凝血酶时间延长，红细胞渗透脆性增加，肝损伤,血脂异常,给予CCl4大鼠的氧化应激和脂质过氧化。如从CCl4和S+CCl4大鼠之间的比较所观察到的,水飞蓟素减弱了大多数这些作用。这项研究的结果表明，水飞蓟素预处理可以减轻CCl4引起的Wistar大鼠肝毒性对凝血功能和红细胞渗透脆性的破坏。
