UNASSIGNED: Lung samples were collected after exposure of Balb/c mice to e-cigarette aerosols for 1 h/day (6 times/week) for 1, 2 and 4 weeks and compared to sham-exposed controls. Examined biomarkers including inflammatory cells, tumor necrosis factor α (TNFα), interleukin-6 (IL-6), interleukin-10 (IL-10), reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), catalase, superoxide dismutase (SOD), and Thiobarbituric acid reactive substances (TBARS).
UNASSIGNED: Exposure of animals to e-cigarette aerosols induced significant increases (P < 0.05) in total inflammatory cells, eosinophils, macrophages and TNFα in the lung tissue after 1, 2 and 4 weeks of exposure. Furthermore, level of IL-10 significantly decreased, whereas levels of neutrophils and basophils significantly increased (P < 0.05) after 1 week of exposure. Exposure of animals to e-cigarette aerosol also induced significant decreases (P < 0.05) in the GSH/GSSG ratio, and GPx levels after 2 and 4 weeks of exposures. The activity of catalase was also reduced (P < 0.05) after 4 weeks of exposure. Level of TBARS showed a trend of elevation with time and it reached a significant elevation after 4 weeks (P < 0.01).
UNASSIGNED: Current results indicate that inhalation of unflavored e-cigarette aerosol might be associated with inflammation in lung tissue that worsen as the duration of exposure increases. Further experiments including more time points, histopathology and pulmonary physiology experiments are needed to confirm the current results.
未经证实:在Balb/c小鼠暴露于电子烟气雾剂1小时/天(6次/周)1、2和4周后收集肺样品,并与假暴露对照进行比较。检查的生物标志物,包括炎症细胞,肿瘤坏死因子α(TNFα),白细胞介素-6(IL-6),白细胞介素-10(IL-10),还原型谷胱甘肽(GSH),氧化型谷胱甘肽(GSSG),谷胱甘肽过氧化物酶(GPx),过氧化氢酶,超氧化物歧化酶(SOD),和硫代巴比妥酸反应性物质(TBARS)。
UNASSIGNED:动物暴露于电子烟气雾剂诱导总炎性细胞显著增加(P<0.05),嗜酸性粒细胞,暴露1、2和4周后肺组织中的巨噬细胞和TNFα。此外,IL-10水平显著下降,而嗜中性粒细胞和嗜碱性粒细胞在暴露1周后显著升高(P<0.05)。动物暴露于电子烟气溶胶也导致GSH/GSSG比率显着降低(P<0.05),暴露2周和4周后的GPx水平。接触4周后过氧化氢酶活性也降低(P<0.05)。TBARS水平随时间呈升高趋势,4周后达到显著升高(P<0.01)。
UNASSIGNED:目前的结果表明,吸入未加味的电子烟气雾剂可能与肺组织炎症有关,随着暴露时间的增加,炎症会恶化。进一步的实验包括更多的时间点,需要进行组织病理学和肺生理学实验来证实目前的结果。