通过使用金纳米颗粒(AuNPs)的表面增强拉曼光谱(SERS)已与生物分子和某些癌细胞的鉴定相关。寻找更绿色的NP合成替代品,我们评估了使用不同丝状真菌产生的AuNPs的SERS特性。利用灰葡萄孢菌的上清液合成AuNPs,atroviride木霉,木霉,Alternariasp.和无柄灵芝。通过紫外-可见光谱(UV-Vis)对AuNPs进行表征,以确定其特征性的表面等离子体共振,位于545nm(B.cinerea),550nm(T.atroviride),540nm(T.天蚕),530nm(链格孢菌sp。),和525nm(G.无柄)。形态学,通过透射电子显微镜(TEM)表征尺寸和晶体结构;通过Z电位测量评估胶体稳定性。我们发现,在特定的孵育条件下,有可能获得球形和准球形的AuNP,其平均大小范围取决于92.9nm的真菌物种上清液(B.cinerea),24.7nm(T.atroviride),16.4nm(T.天蚕),9.5纳米(链格孢菌。),和13.6nm(G.无柄)。这个,正如可以预期的那样,对拉曼放大有影响。在532nm波长下操作的显微拉曼光谱系统用于评估AuNP的SERS特征。我们选择亚甲基蓝作为我们的靶分子,因为它已在文献中广泛用于这样的目的。我们的结果表明,AuNPs与T.atroviride的上清液合成,天蚕和链格孢菌。产生更强的SERS效应,增强因子(EF)分别为20.9、28.8和35.46。这些结果是有希望的,可以作为开发生物传感器的基线,简单,和低成本的绿色替代品。
Surface enhanced Raman spectroscopy (
SERS) by using gold nanoparticles (AuNPs) has gained relevance for the identification of biomolecules and some cancer cells. Searching for greener NPs synthesis alternatives, we evaluated the
SERS properties of AuNPs produced by using different filamentous fungi. The AuNPs were synthesized utilizing the supernatant of Botrytis cinerea, Trichoderma atroviride, Trichoderma asperellum, Alternaria sp. and Ganoderma sessile. The AuNPs were characterized by ultraviolet-visible spectroscopy (UV-Vis) to identify its characteristic surface plasmon resonance, which was located at 545 nm (B. cinerea), 550 nm (T. atroviride), 540 nm (T. asperellum), 530 nm (Alternaria sp.), and 525 nm (G. sessile). Morphology, size and crystal structure were characterized through transmission electron microscopy (TEM); colloidal stability was assessed by Z-potential measurements. We found that, under specific incubation conditions, it was possible to obtain AuNPs with spherical and quasi-spherical shapes, which mean size range depends on the fungal species supernatant with 92.9 nm (B. cinerea), 24.7 nm (T. atroviride), 16.4 nm (T. asperellum), 9.5 nm (Alternaria sp.), and 13.6 nm (G. sessile). This, as it can be expected, has an effect on Raman amplification. A micro-Raman spectroscopy system operated at a wavelength of 532 nm was used for the evaluation of the
SERS features of the AuNPs. We chose methylene blue as our target molecule since it has been widely used for such a purpose in the literature. Our results show that AuNPs synthesized with the supernatant of T. atroviride, T. asperellum and Alternaria sp. produce the stronger
SERS effect, with enhancement factor (EF) of 20.9, 28.8 and 35.46, respectively. These results are promising and could serve as the base line for the development of biosensors through a facile, simple, and low-cost green alternative.