Abstract：
The engineering of a home-made portable double-layer filtration and concentration device with the common syringe for rapid analysis of water samples is reported. The core elements of the device were two installed filtration membranes with different pore sizes for respective functions. The upper filtration membrane was used for preliminary intercepting large interfering impurities (interception membrane), while the lower filtration membrane was used for collecting multiple target pathogens (enrichment membrane) for determination. This combination can make the contaminated environmental water, exemplified by surface water, filtrated quickly through the device and just retained the target bacteria of Escherichia coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes on the lower enrichment membrane. Integrating with surface-enhanced Raman spectra (SERS) platform to decode the SERS-Tags (SERS-TagCVa, SERS-TagR6G, and SERS-TagMB) already labeled on each of the enriched bacteria based the antibody-mediated immuno-recognition effect, fast separation, concentration, and detection of multiple pathogenic bacteria from the bulk of contaminated environmental water were realized. Results show that within 30 min, all target bacteria in the lake water can be simultaneously and accurately measured in the range from 101 to 106 CFU mL-1 with detection limit of 10.0 CFU mL-1 without any pre-culture procedures. This work highlights the simplicity, rapidness, cheapness, selectivity, and the robustness of the constructed method for simultaneous detecting multiple pathogens in aqueous samples. This protocol opens a new avenue for facilitating the development of versatile analytical tools for drinking water and food safety monitoring in underdeveloped or developing countries.
摘要：
报道了一种家用便携式双层过滤和浓缩装置的工程，该装置带有普通注射器，用于快速分析水样。该装置的核心元件是两个安装的过滤膜,对于各自的功能具有不同的孔径。上层过滤膜用于初步拦截大的干扰杂质(拦截膜),而下滤膜用于收集多个目标病原体（富集膜）进行测定。这种组合可以使被污染的环境水，以地表水为例，通过设备快速过滤，并保留了目标细菌大肠杆菌O157：H7，金黄色葡萄球菌，和单增李斯特菌在下部富集膜上。与表面增强拉曼光谱（SERS）平台集成以解码SERS标签（SERS-TagCVa，SERS-TagR6G,和SERS-TagMB）已经基于抗体介导的免疫识别作用标记在每种富集细菌上，快速分离,浓度,并实现了对大量污染环境水中多种致病菌的检测。结果表明，在30分钟内，湖水中的所有目标细菌可以在101至106CFUmL-1的范围内同时准确地测量，检出限为10.0CFUmL-1，无需任何预培养程序。这项工作突出了简单性，快速，廉价，选择性,以及所构建的同时检测水性样品中多种病原体的方法的鲁棒性。该协议为促进开发不发达国家或发展中国家饮用水和食品安全监管的通用分析工具开辟了新的途径。
