PDF(Pubmed)
Abstract：
UNASSIGNED: Insulin and C-peptide played crucial roles as clinical indicators for diabetes and certain liver diseases. However, there has been limited research on the simultaneous detection of insulin and C-peptide in trace serum. It is necessary to develop a novel method with high sensitivity and specificity for detecting insulin and C-peptide simultaneously.
UNASSIGNED: A core-shell-satellites hierarchical structured nanocomposite was fabricated as SERS biosensor using a simple wet-chemical method, employing 4-MBA and DTNB for recognition and antibodies for specific capture. Gold nanorods (Au NRs) were modified with Raman reporter molecules and silver nanoparticles (Ag NPs), creating SERS tags with high sensitivity for detecting insulin and C-peptide. Antibody-modified commercial carboxylated magnetic bead@antibody served as the capture probes. Target materials were captured by probes and combined with SERS tags, forming a \"sandwich\" composite structure for subsequent detection.
UNASSIGNED: Under optimized conditions, the nanocomposite fabricated could be used to detect simultaneously for insulin and C-peptide with the detection limit of 4.29 × 10-5 pM and 1.76 × 10-10 nM in serum. The insulin concentration (4.29 × 10-5-4.29 pM) showed a strong linear correlation with the SERS intensity at 1075 cm-1, with high recoveries (96.4-105.3%) and low RSD (0.8%-10.0%) in detecting human serum samples. Meanwhile, the C-peptide concentration (1.76 × 10-10-1.76 × 10-3 nM) also showed a specific linear correlation with the SERS intensity at 1333 cm-1, with recoveries 85.4%-105.0% and RSD 1.7%-10.8%.
UNASSIGNED: This breakthrough provided a novel, sensitive, convenient and stable approach for clinical diagnosis of diabetes and certain liver diseases. Overall, our findings presented a significant contribution to the field of biomedical research, opening up new possibilities for improved diagnosis and monitoring of diabetes and liver diseases.
UNASSIGNED: A core-shell-satellites hierarchical structured nanocomposite was fabricated as SERS biosensor using a simple wet-chemical method, employing 4-MBA and DTNB for recognition and antibodies for specific capture. Gold nanorods (Au NRs) were modified with Raman reporter molecules and silver nanoparticles (Ag NPs), creating SERS tags with high sensitivity for detecting insulin and C-peptide. Antibody-modified commercial carboxylated magnetic bead@antibody served as the capture probes. Target materials were captured by probes and combined with SERS tags, forming a \"sandwich\" composite structure for subsequent detection.
UNASSIGNED: Under optimized conditions, the nanocomposite fabricated could be used to detect simultaneously for insulin and C-peptide with the detection limit of 4.29 × 10-5 pM and 1.76 × 10-10 nM in serum. The insulin concentration (4.29 × 10-5-4.29 pM) showed a strong linear correlation with the SERS intensity at 1075 cm-1, with high recoveries (96.4-105.3%) and low RSD (0.8%-10.0%) in detecting human serum samples. Meanwhile, the C-peptide concentration (1.76 × 10-10-1.76 × 10-3 nM) also showed a specific linear correlation with the SERS intensity at 1333 cm-1, with recoveries 85.4%-105.0% and RSD 1.7%-10.8%.
UNASSIGNED: This breakthrough provided a novel, sensitive, convenient and stable approach for clinical diagnosis of diabetes and certain liver diseases. Overall, our findings presented a significant contribution to the field of biomedical research, opening up new possibilities for improved diagnosis and monitoring of diabetes and liver diseases.
摘要：
■胰岛素和C肽作为糖尿病和某些肝病的临床指标起着至关重要的作用。然而,关于同时检测微量血清中胰岛素和C肽的研究有限。有必要开发一种具有高灵敏度和特异性的新方法来同时检测胰岛素和C肽。
■使用简单的湿化学方法制造了核-壳-卫星分层结构的纳米复合材料作为SERS生物传感器，采用4-MBA和DTNB进行识别，抗体进行特异性捕获。金纳米棒(AuNRs)用拉曼报道分子和银纳米粒子(AgNP)修饰,建立高灵敏度的SERS标签，用于检测胰岛素和C肽。抗体修饰的商业羧化磁珠@抗体用作捕获探针。通过探针捕获目标材料并结合SERS标签，形成“三明治”复合结构，用于后续检测。
■在优化条件下，制备的纳米复合材料可用于同时检测胰岛素和C肽，检出限为4.29×10-5pM和1.76×10-10nM。胰岛素浓度（4.29×10-5-4.29pM）与1075cm-1处的SERS强度呈强线性相关，在检测人血清样品中具有高回收率（96.4-105.3％）和低RSD（0.8％-10.0％）。同时,C肽浓度（1.76×10-10-1.76×10-3nM）也与1333cm-1处的SERS强度呈特定的线性相关，回收率为85.4％-105.0％，RSD为1.7％-10.8％。
■这一突破提供了一种小说，敏感,方便，稳定的方法，用于糖尿病和某些肝病的临床诊断。总的来说,我们的发现为生物医学研究领域做出了重大贡献，为改善糖尿病和肝病的诊断和监测开辟了新的可能性。
■使用简单的湿化学方法制造了核-壳-卫星分层结构的纳米复合材料作为SERS生物传感器，采用4-MBA和DTNB进行识别，抗体进行特异性捕获。金纳米棒(AuNRs)用拉曼报道分子和银纳米粒子(AgNP)修饰,建立高灵敏度的SERS标签，用于检测胰岛素和C肽。抗体修饰的商业羧化磁珠@抗体用作捕获探针。通过探针捕获目标材料并结合SERS标签，形成“三明治”复合结构，用于后续检测。
■在优化条件下，制备的纳米复合材料可用于同时检测胰岛素和C肽，检出限为4.29×10-5pM和1.76×10-10nM。胰岛素浓度（4.29×10-5-4.29pM）与1075cm-1处的SERS强度呈强线性相关，在检测人血清样品中具有高回收率（96.4-105.3％）和低RSD（0.8％-10.0％）。同时,C肽浓度（1.76×10-10-1.76×10-3nM）也与1333cm-1处的SERS强度呈特定的线性相关，回收率为85.4％-105.0％，RSD为1.7％-10.8％。
■这一突破提供了一种小说，敏感,方便，稳定的方法，用于糖尿病和某些肝病的临床诊断。总的来说,我们的发现为生物医学研究领域做出了重大贡献，为改善糖尿病和肝病的诊断和监测开辟了新的可能性。
