Abstract：
BACKGROUND: The application of direct current can have a significant impact on the rate of tooth movement and surrounding periodontal ligament collagen turnover. This study aims to provide insight into the optimal characteristics of applied current to achieve enhanced tissue response.
METHODS: Eighteen male Wistar rats were divided into three groups (I, II, and III). Split mouth design was used, and each side was allocated into an experimental group or control group. Experimental sides of groups I, II, and III received 20, 10, and 15 μA of current (15 min, twice daily for 3 days). Both the experimental and control groups receive an orthodontic force via the NiTi closed coil spring. The amount of tooth movement was determined daily. Immunohistochemistry slides were scored using the immunoreactive scoring (IRS) system for collagen types I and III. One-way Analysis of Variance (ANOVA) and Tukey post hoc test were used to analyse the rate of tooth movement, while Mann-Whitney test was used to analyse IRS distribution between control and experimental groups.
RESULTS: Compared with the control group, there was a statistically significant difference in tooth movement in all the experimental groups, with group 3 showing the maximum rate on days 2 and 3. This was supported by immunoreactive scores for both collagen types I and III.
CONCLUSIONS: After 72 hours, the expression of collagen types 1 and 3 increased significantly for group III. This finding was in harmony with the rate of tooth movement, which was maximum for group 3 (15 μA) as compared to other groups.
METHODS: Eighteen male Wistar rats were divided into three groups (I, II, and III). Split mouth design was used, and each side was allocated into an experimental group or control group. Experimental sides of groups I, II, and III received 20, 10, and 15 μA of current (15 min, twice daily for 3 days). Both the experimental and control groups receive an orthodontic force via the NiTi closed coil spring. The amount of tooth movement was determined daily. Immunohistochemistry slides were scored using the immunoreactive scoring (IRS) system for collagen types I and III. One-way Analysis of Variance (ANOVA) and Tukey post hoc test were used to analyse the rate of tooth movement, while Mann-Whitney test was used to analyse IRS distribution between control and experimental groups.
RESULTS: Compared with the control group, there was a statistically significant difference in tooth movement in all the experimental groups, with group 3 showing the maximum rate on days 2 and 3. This was supported by immunoreactive scores for both collagen types I and III.
CONCLUSIONS: After 72 hours, the expression of collagen types 1 and 3 increased significantly for group III. This finding was in harmony with the rate of tooth movement, which was maximum for group 3 (15 μA) as compared to other groups.
摘要：
背景：直流电的应用会对牙齿移动速度和周围牙周膜胶原周转产生重大影响。本研究旨在深入了解施加电流的最佳特性，以实现增强的组织反应。
方法：18只雄性Wistar大鼠分为三组（I，II,andIII).使用了裂口设计，每侧分为实验组或对照组。第一组的实验侧，II,III接受20、10和15μA的电流(15分钟，每天两次，共3天）。实验组和对照组都通过NiTi闭合螺旋弹簧接收正畸力。每天确定牙齿移动的量。使用免疫反应性评分（IRS）系统对I型和III型胶原蛋白进行免疫组织化学切片评分。单因素方差分析（ANOVA）和Tukey事后检验用于分析牙齿移动的速度，而Mann-Whitney检验用于分析对照组和实验组之间的IRS分布。
结果：与对照组相比，所有实验组的牙齿移动有统计学上的显著差异，第3组显示第2天和第3天的最大比率。这得到I型和III型胶原的免疫反应性评分的支持。
结论：72小时后，III组1型和3型胶原蛋白的表达显着增加。这一发现与牙齿移动的速度一致，与其他组相比，第3组（15μA）最大。
方法：18只雄性Wistar大鼠分为三组（I，II,andIII).使用了裂口设计，每侧分为实验组或对照组。第一组的实验侧，II,III接受20、10和15μA的电流(15分钟，每天两次，共3天）。实验组和对照组都通过NiTi闭合螺旋弹簧接收正畸力。每天确定牙齿移动的量。使用免疫反应性评分（IRS）系统对I型和III型胶原蛋白进行免疫组织化学切片评分。单因素方差分析（ANOVA）和Tukey事后检验用于分析牙齿移动的速度，而Mann-Whitney检验用于分析对照组和实验组之间的IRS分布。
结果：与对照组相比，所有实验组的牙齿移动有统计学上的显著差异，第3组显示第2天和第3天的最大比率。这得到I型和III型胶原的免疫反应性评分的支持。
结论：72小时后，III组1型和3型胶原蛋白的表达显着增加。这一发现与牙齿移动的速度一致，与其他组相比，第3组（15μA）最大。
