■磷脂酶A2(PLA2)是参与炎症过程的一大类酶,可催化膜磷脂的水解,导致游离脂肪酸和溶血磷脂的产生,开始花生四烯酸级联反应.它们的表达与几种癌症的行为有关。我们的目标是寻找与预后和生存相关的前列腺癌(PCa)组织中PLA2的表达。
■使用qRT-PCR,我们分析了PLA2G1B的表达水平,PLA2G2A,PLA2G2D,PLA2G4A,PLA2G4B,PLA2G4C,PLA2G4D,PLA2G4E,PLA2G4F,108例前列腺癌根治术患者PCa组织中的PLA2G6、PLA2G7、PLA2G16、PNPLA1和PNPLA2,其次是平均时间163个月。
■与正常组织相比,所有PLA2在PCa中过表达。有趣的是,某些PLA2的高表达与有利的预后因素有关:PSA水平较低(PLA2G2A,PLA2G4D),淋巴结转移率较低(PLA2G16和PLA2G1B),和器官局限性疾病(PLA2G4A)。最重要的是,PLAG4B与更长的无病生存期独立相关。
■这是第一个全面探索PLA2在PCa中表达水平的研究,表明某些PLA2的较高表达应用作预后良好和无病生存期更长的生物标志物。
UNASSIGNED: Phospholipase A2 (PLA2) is a large family of enzymes involved in the inflammatory process that catalyzes the hydrolysis of membrane phospholipids, leading to the production of free fatty acids and lysophospholipids, starting the arachidonic acid cascade. Their expression has been related to the behavior of several cancers. Our objective is to search for PLA2 expression in prostate cancer (PCa) tissue that correlates with prognosis and survival.
UNASSIGNED: Using qRT-PCR, we analyzed the expression levels of PLA2G1B, PLA2G2A, PLA2G2D, PLA2G4A, PLA2G4B, PLA2G4C, PLA2G4D, PLA2G4E, PLA2G4F, PLA2G6, PLA2G7, PLA2G16, PNPLA1, and PNPLA2 in PCa tissue from 108 patients submitted to radical prostatectomy, followed by a mean time of 163 months.
UNASSIGNED: All PLA2 was overexpressed in PCa compared to normal tissue. Interestingly, higher expression of some PLA2 was related to favorable prognostic factors: lower levels of PSA (PLA2G2A, PLA2G4D), lower rates of lymph node metastasis (PLA2G16 and PLA2G1B), and organ-confined disease (PLA2G4A). Most importantly, PLAG4B was independently related to longer disease-free survival.
UNASSIGNED: This is the first study exploring comprehensively the expression levels of PLA2 in PCa, showing that the higher expression of some PLA2 should be used as biomarkers of good prognosis and longer disease-free survival.