在控制植物病害方面,生物防治剂比化学品更受欢迎,木霉属物种对土壤传播的病原体特别有效。这项研究检查了使用高度拮抗菌株,木霉菌A10和一种强毒株,通过ITS鉴定和确认的菌核Sr38,β-微管蛋白(T.天蚕),TEF1α,和RPB2(S.rolfsii)序列。体外和植物实验比较了A10与其他拮抗真菌和杀真菌剂对S.rolfsii的拮抗潜力。在双重培养测定中,A10实现了对S.rolfsii的94.66%抑制。在番茄品种PusaRuby的温室试验中,A10显示出显着的接种前和接种后有效性,疾病抑制分别为86.17和80.60%,分别,胜过T.harzianum,丙环唑,和多菌灵.此外,探索了用A10引发微生物以增强植物防御反应。用天蚕A10预处理番茄植株导致几种防御相关基因的显着上调,包括PR1、PR2、PR3、PR5、PR12、硫氧还蛋白过氧化物酶、过氧化氢酶,多酚氧化酶,苯丙氨酸氨裂解酶,异氯酸盐合成酶,漆酶,prosystemin,多重抑制素,WRKY31,MYC2,脂氧合酶A,脂氧合酶C,蛋白酶抑制剂I,蛋白酶抑制剂II,和乙烯反应1与各种信号途径相关,例如水杨酸(SA)介导的和茉莉酸/乙烯(JA/ET)介导的反应。在接种S.rolfsii攻击的A10引发的植物中,这种上调在接种后48小时特别明显,诱导对领腐病的抗性。这项研究强调了天蚕A10在控制颈圈腐烂中的有效性,并强调了其通过微生物引发诱导植物抗性的潜力,为可持续疾病管理策略提供有价值的见解。
■在线版本包含补充材料,可在10.1007/s13205-024-04040-4获得。
Biological control agents are preferred over chemicals for managing plant diseases, with Trichoderma species being particularly effective against soil-borne pathogens. This study examines the use of a highly antagonistic strain, Trichoderma asperellum A10, and a virulent strain, Sclerotium rolfsii Sr38, identified and confirmed through ITS, β-tubulin (T. asperellum), TEF 1α, and RPB2 (S. rolfsii) sequences. In vitro and in planta experiments compared the antagonistic potential of A10 with other antagonistic fungi and fungicides against S. rolfsii. A10 achieved 94.66% inhibition of S. rolfsii in dual culture assays. In greenhouse trials with tomato variety Pusa Ruby, A10 showed significant pre- and post-inoculation effectiveness, with disease inhibition of 86.17 and 80.60%, respectively, outperforming T. harzianum, Propiconazole, and Carbendazim. Additionally, microbial priming with A10 was explored to enhance plant defense responses. Pre-treatment of tomato plants with T. asperellum A10 led to significant upregulation of several defense-related genes, including PR1, PR2, PR3, PR5, PR12, thioredoxin peroxidase, catalase, polyphenol oxidase, phenylalanine ammonia lyase, isochorismate synthase, laccase, prosystemin, multicystatin, WRKY31, MYC2, lipoxygenase A, lipoxygenase C, proteinase inhibitor I, proteinase inhibitor II, and ethylene response 1 associated with various signaling pathways such as salicylic acid (SA)-mediated and jasmonic acid/ethylene (JA/ET)-mediated responses. This upregulation was particularly evident at 48 h post-inoculation in A10-primed plants challenged with S. rolfsii, inducing resistance against collar rot disease. This study underscores the effectiveness of T. asperellum A10 in controlling collar rot and highlights its potential for inducing resistance in plants through microbial priming, providing valuable insights into sustainable disease management strategies.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-024-04040-4.