■骨质疏松症是一个主要的健康问题。MicroRNAs(miRNAs)在调节细胞生长和发育中起着多种作用。高通量测序技术目前应用广泛。
■筛选和验证与骨质疏松症相关的miRNA。
■收集患有(n=3)和没有(n=3)骨质疏松症的患者的骨标本。高通量测序用于筛选miRNA,然后使用火山图进行分析,韦恩地图,基因本体论(GO)分析,和京都基因和基因组百科全书(KEGG)分析。使用qRT-PCR进行miRNA的确认。
■测序分析表明,在骨质疏松症中有12个miRNA下调,有5个miRNA上调。GO和KEGG将这些miRNA鉴定为与骨代谢相关。qRT-PCR结果显示,miR-140-5p,miR-127-3p,miR-199b-5p,miR-181a-5p,miR-181d-5p,和miR-542-3p表现出2.27-的下降,3.00-,3.48-,2.67-,2.41-,与对照组相比,骨质疏松症为1.98倍(所有P<0.05)。相反,miR-486-3p和miR-486-5p分别增加2.17和3.89倍(P<0.05)(均P<0.05)。
这项研究利用高通量测序来检测在患有骨质疏松症的个体中不同表达的miRNA。在骨质疏松症中,六个miRNA(miR-140-5p,miR-127-3p,miR-199b-5p,miR-181a-5p,miR-181d-5p,和miR-542)被发现表达降低,而两个miRNA(miR-486-3p和miR-486-5p)被发现表达增加。各种miRNA的初始表现可能作为预测指标,并可能预测骨质疏松症的进展。
UNASSIGNED: Osteoporosis is a major health issue. MicroRNAs (miRNAs) play multiple roles in regulating cell growth and development. High-throughput sequencing technology is widely used nowadays.
UNASSIGNED: To screen for and validate miRNAs associated with osteoporosis.
UNASSIGNED: Bone specimens from patients with (n = 3) and without (n = 3) osteoporosis were collected. High-throughput sequencing was used to screen for miRNAs that were then analyzed using volcano maps, Wayne maps, gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Confirmation of the miRNAs was done using qRT-PCR.
UNASSIGNED: The analysis of sequencing showed that there were 12 miRNAs that were down-regulated and five miRNAs that were upregulated in osteoporosis. GO and KEGG identified these miRNAs as being associated with bone metabolism. The qRT-PCR results showed that miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542-3p exhibited a decrease of 2.27-, 3.00-, 3.48-, 2.67-, 2.41-, and 1.98-fold (all P < 0.05) in osteoporosis compared to controls. Conversely, miR-486-3p and miR-486-5p demonstrated an increase of 2.17- and 3.89-fold (P < 0.05) (all P < 0.05).
UNASSIGNED: This study utilized high-throughput sequencing to detect miRNAs that were expressed differently in individuals with osteoporosis. In osteoporosis, six miRNAs (miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542) were found to have decreased expression, whereas two miRNAs (miR-486-3p and miR-486-5p) were found to have increased expression. The initial manifestation of various miRNAs might serve as predictive indicators and potentially anticipate the progression of osteoporosis.